Adiponectin regulates ovarian cancer cell proliferation,migration,and invasion via the PI3K/Akt/mTOR signaling pathway
10.3969/j.issn.1673-4130.2025.19.003
- VernacularTitle:脂联素通过PI3K/Akt/mTOR信号通路调控卵巢癌细胞增殖、迁移和侵袭行为
- Author:
Yixin LIN
1
;
Xiaoqian ZHU
;
Xinger WU
;
Kang CHEN
Author Information
1. 广东医科大学第一临床医学院,广东湛江 524023;中山市人民医院检验医学中心,广东中山 528403
- Keywords:
ovarian cancer;
adiponectin;
cell proliferation;
cell invasion;
phosphatidylinositol 3-ki-nase/protein kinase B/mammalian target of rapamycin signal axis
- From:
International Journal of Laboratory Medicine
2025;46(19):2319-2325
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of adiponectin(APN)on the proliferation,migration,and invasion of ovarian cancer cells and its potential association with the phosphatidylinositol 3-kinase(PI3K)/pro-tein kinase B(Akt)/mammalian target of rapamycin(mTOR)signaling pathway.Methods A2780 and SK-OV3 cell lines were selected.AdipoR1 mRNA expression was detected by real-time polymerase chain reaction(RT-PCR).The CCK-8 assay was employed to evaluate APN-induced proliferation,while scratch wound heal-ing and Transwell assays were conducted to assess migration and invasion capabilities.Western blot analysis was performed to measure the expression levels of PI3K,Akt,mTOR,and their phosphorylated forms(p-Akt,p-mTOR)at different time points(0-60 min)after APN treatment and across intervention groups(APN,LY294002,APN+LY294002).Results Both A2780 and SKOV3 cells expressed AdipoR1 mRNA.Compared with the control group,APN significantly enhanced the proliferation,migration,and invasion of o-varian cancer cells(P＜0.05).Under the stimulation of APN,the expression levels of p-Akt and p-mTOR in A2780 and SKOV3 cells showed a significant upward trend.The peak expression of p-Akt and p-mTOR in A2780 cells was observed at 30 minutes,while in SKOV3 cells,it occurred at 60 minutes.The expression lev-els of p-Akt and p-mTOR were significantly reduced in the LY294002 group and APN+LY294002 group(P＜0.001).Conclusion APN enhances the proliferation,migration,and invasion behavior of ovarian cancer cells by activating the PI3K/Akt/mTOR signaling pathway,and its effect can be reversed by PI3K inhibitors,providing a theoretical basis for targeted intervention in ovarian cancer progression.
