Advances in metagenomics next-generation sequencing in bone and joint infections
10.3969/j.issn.1673-4130.2025.17.020
- VernacularTitle:宏基因组二代测序在骨关节感染中的研究进展
- Author:
Zihan FENG
1
;
Feng PANG
Author Information
1. 山东第二医科大学医学检验学院,山东 潍坊 261053
- Keywords:
metagenomics next-generation sequencing;
bone and joint infections;
diagnosis
- From:
International Journal of Laboratory Medicine
2025;46(17):2158-2162
- CountryChina
- Language:Chinese
-
Abstract:
Bone and joint infections(BJI)are serious postoperative complications,and traditional bacterial culture techniques serve as the gold standard for diagnosis,but there are problems with untimely or undiag-nosed diagnosis.Metagenomics next-generation sequencing(mNGS),as an emerging technology,can detect various pathogens such as bacteria,viruses,parasites,and fungi in samples without bias,providing a new ap-proach for the diagnosis of BJI.This article reviews the application progress of mNGS in BJI,including techni-cal principles,advantages,limitations,and clinical application status.mNGS exhibits high sensitivity and speci-ficity in the diagnosis of prosthetic joint infections(PJI),and its advantages are obvious especially in cultiva-ting negative cases.In addition,mNGS can optimize traditional culture methods,improve the sensitivity of in-traoperative culture,and be used to guide antibiotic selection.However,mNGS has limitations such as slow database updates,sample contamination,and high costs.Further research is needed in the future to elucidate its complete clinical benefits and promote its widespread application in the field of orthopedics.
