Inhibition effect of secondary metabolites of Pseudomonas aeruginosa on Candida albicans and machanisms explore in vitro
10.3969/j.issn.1673-4130.2025.17.009
- VernacularTitle:铜绿假单胞菌次生代谢产物对体外白念珠菌抑制效果和机制探究
- Author:
Peng WANG
1
;
Yuhang LUO
;
Ping QIU
;
Qi LI
;
Jiawei LIU
;
Linjuan CHEN
;
Xuan CHEN
;
Weihong WEN
;
Lingqing XU
Author Information
1. 广州医科大学附属清远医院(清远市人民医院):皮肤科,广东 清远 511518
- Keywords:
Pseudomonas aeruginosa;
Candida albicans;
secondary metabolites;
phenazine;
inhibi-tory effect
- From:
International Journal of Laboratory Medicine
2025;46(17):2097-2104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect of secondary metabolites of Pseudomonas aeruginosa(PA)on Candida albicans(CA)and to explore some of the mechanisms.Methods PA and CA strains were i-solated from clinical specimens from the hospital.Then,PA strains with inhibitory effects on CA were screened through cross-line test and co-incubation test,and crude extracts of PA secondary metabolites were prepared,and were tested together with pyocyanin,phenazine-1-carboxylic acid,1-hydroxyphenazine,and 3-ox-ododecyl-l-homoserine lactone(3-oxo-HSL).The inhibitory effects of various PA secondary metabolites on CA were determined through minimum inhibitory concentration test,minimum bactericidal concentration test,time-sterilization curve measurement,and XTT method activity measurement test,and some mechanisms by which PA secondary metabolites inhibited CA were explored.Results The strongest inhibitory effect on CA was 1-hydroxyphenazine,and at a concentration of 6.250 μg/mL,the relative activity of CA decreased to 0.00%.Next were pyocyanin and PA crude extract,and the relative fungal activity of CA decreased to 0.00%at concentrations of 200 and 100 μg/mL.1-hydroxyphenazine,pyocyanin,3-oxo-HSL and PA crude extract all had inhibitory effects on the formation of CA hyphae.Reactive oxygen species(ROS)were generated in CA cells treated with 1-hydroxyphenazine,phenazine 1-carboxylic acid,pyocyanin,and PA crude extract,and the highest levels of ROS were induced by pyocyanin and 1-hydroxyphenazine.Conclusion Phenazine secondary metabolites 1-hydroxyphenazine and pyocyanin have significant inhibitory effects on the growth and activity of CA,and both induce the highest amount of ROS.The quorum-sensing signal molecule 3-oxo-HSL have no in-hibitory effect on CA growth,but have a significant inhibitory effect on the formation of fungal hyphae.
