Expression and clinical significance of HSPA5 and APOBEC3B in high-risk HPV-positive cervical cancer patients
10.3969/j.issn.1673-4130.2025.16.002
- VernacularTitle:高危型HPV阳性宫颈癌患者中HSPA5、APOBEC3B的表达及临床意义研究
- Author:
Yuhao LU
1
;
Yanfen GUAN
;
Jianmin WU
Author Information
1. 珠海市中西医结合医院病理科,广东珠海 519020
- Keywords:
human papillomavirus;
cervical cancer;
heat shock protein family A member 5;
apoli-poprotein B mRNA editing enzyme catalytic polypeptide-like protein 3B;
prognosis
- From:
International Journal of Laboratory Medicine
2025;46(16):1927-1932
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical significance of heat shock protein family A member 5(HSPA5)and apolipoprotein B mRNA editing enzyme catalytic polypeptide-like protein 3B(APO-BEC3B)in high-risk human papillomavirus(HR-HPV)positive cervical cancer patients.Methods A total of 86 patients with HR-HPV positive cervical cancer tissues diagnosed and treated in Zhuhai Integrated Tradi-tional Chinese and Western Medicine Hospital from March 2019 to March 2021 were retrospectively collected as the case group,50 cases of HR-HPV negative cervical cancer patients tissues diagnosed and treated during the same period were selected as the case control group,and 50 cases of normal cervical tissues from patients undergoing total hysterectomy were selected as the benign control group during the same period.The protein expressions of HSPA5 and APOBEC3B were analyzed by immunohistochemistry.The expressions of HSPA5 mRNA and APOBEC3B mRNA were detected by real-time fluorescence quantitative PCR.Kaplan-Meier sur-vival curve was used for survival analysis,and Cox proportional hazards regression model was used to analyze the prognostic factors of HR-HPV positive cervical cancer patients.Results The expressions of HSPA5 mR-NA and APOBEC3B mRNA in the case group were higher than that in the case control group and benign con-trol group,and the difference was statistically significant(P＜0.05).The positive expressions of HSPA5 pro-tein and APOBEC3B protein in the case group were higher than those in the case control group and the benign control group,and the difference was statistically significant(P＜0.05).Compared with FIGO stage Ⅰ A-Ⅰ B and without lymph node metastasis,the positive rates of HSPA5 and APOBEC3B proteins in HR-HPV positive cancer tissues with FIGO stage Ⅱ A and lymph node metastasis were higher,and the difference was statistically significant(P＜0.05).The 3-year survival rate of HSPA5 protein positive group was 68.52％(37/54),which was lower than that of HSPA5 protein negative group(87.50％,28/32),and the difference was statistically significant(Log Rank x2=4.103,P=0.043).The 3-year survival rate of APOBEC3B protein positive group was 67.31％(35/52),which was lower than that of APOBEC3B protein negative group(88.24％,30/34),and the difference was statistically significant(Log Rank x2=5.241,P=0.022).FIGO stage(HR=1.570,95％CI:1.038-2.374),lymph node metastasis(HR=1.754,95％CI:1.109-2.775)and HSPA5 protein(HR=1.616,95％CI:1.154-2.265)and APOBEC3B protein(HR=1.449,95％CI:1.095-1.918)in cancer tissues were independent factors affecting the prognosis of HR-HPV positive cervical cancer(P＜0.05).Conclusion HSPA5 and APOBEC3B mRNA and protein levels are significantly increased in HR-HPV positive cervical cancer.The protein expression of HSPA5 and APOBEC3B is related to the pro-gression and prognosis of patients with HR-HPV positive cervical cancer,which is a new prognostic marker for evaluating the prognosis of HR-HPV positive cervical cancer.
