Clinical value and relationship of intracellular and plasma HIV RNA load in peripheral blood of HIV-1 patients
10.3969/j.issn.1673-4130.2025.16.001
- VernacularTitle:HIV-1感染者外周血胞内和血浆HIV RNA载量的关系及其临床价值
- Author:
Xiaohan ZHOU
1
;
Juan ZHOU
;
Chuanmin TAO
;
Ying MA
Author Information
1. 四川大学华西医院实验医学科/四川大学华西医院临床检验医学研究中心/四川省医学检验临床医学研究中心,四川成都 610041;潍坊市人民医院检验科,山东潍坊 261000
- Keywords:
intracellular human immunodeficiency virus RNA load;
plasma human immunodeficiency vi-rus RNA load;
antiretroviral therapy
- From:
International Journal of Laboratory Medicine
2025;46(16):1921-1926
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between intracellular HIV RNA load and plasma HIV RNA load in peripheral blood of human immunodeficiency virus(HIV)-1 patients,and to explore the potential clinical value of intracellular HIV RNA load in antiretroviral therapy(ART).Methods A total of 104 HIV-1 patients admitted to the West China Hospital,Sichuan University from April to June 2021 were enrolled,in-cluding 26 untreated patients and 78 treated patients.Peripheral blood was collected and patients were fol-lowed up.The correlation between intracellular HIV RNA load,plasma HIV RNA load and the number of CD4+T lymphocytes during the same period were analyzed.The differences of intracellular HIV RNA load in patients with different plasma HIV RNA load and in patients with different therapeutic effects were com-pared.Results The intracellular HIV RNA load was positively correlated with the plasma HIV RNA load.Both intracellular HIV RNA load and plasma HIV RNA load were negatively correlated with the number of CD4+T lymphocytes.Intracellular HIV RNA load was positive in all patients,among which plasma HIV RNA was negative or below the detection limit in 1 untreated patient and 35 treated patients.There was a statisti-cally significant difference in intracellular HIV RNA load between patients with different plasma viral loads(P＜0.001).Patients with an intracellular HIV RNA load of 3.816 lg copy/106 cells could best distinguish between patients with plasma viral load above the detection limit and below the detection limit[area under the curve(AUC)was 0.738].There was a statistically significant difference in intracellular HIV RNA load be-tween patients with different therapeutic effects(P＜0.000 1),and the intracellular HIV RNA load(3.987 lg copy/106 cells,AUC was 0.985)rather than the plasma HIV RNA load(1.647 lg copy/mL,AUC was 0.854)could better distinguish between virologically suppressed patients and low-level viremia patients.Con-clusion The intracellular HIV RNA load in peripheral blood can reflect the real virus storage level and im-mune damage in HIV-1 patients,and is a promising index to evaluate the efficacy of ART and prognosis of pa-tients.Especially when the plasma viral load was below the detection limit,the monitoring of intracellular HIV RNA load may be a more accurate method for the evaluation of ART efficacy.
