Study on the effects of exposure to various concentrations of cigar smoke on gut microbiota in mice
10.3969/j.issn.1673-4130.2025.14.019
- VernacularTitle:不同雪茄烟气浓度暴露对小鼠肠道菌群的影响研究
- Author:
Jingyuan FAN
1
;
Jing LUO
;
Wanrong HU
;
Yun JIA
;
Xue LIAO
;
Xin WANG
;
Dongliang LI
Author Information
1. 四川中烟工业有限责任公司雪茄烟技术创新中心/烟草行业雪茄发酵工艺重点实验室,四川 成都 610101
- Keywords:
cigar;
smoke;
gut microbiota
- From:
International Journal of Laboratory Medicine
2025;46(14):1770-1777
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of exposure to various concentrations of cigar smoke on gut microbiota in mice.Methods A total of 40 C57BL/6 mice were randomly divided into the control group,the low-dose cigar exposure group,the medium-dose group and the high-dose group,with 10 mice in each group.After 4 weeks of feeding,fecal samples were collected for gene sequencing of 16S ribosomal RNA and analysis of differences in gut microbiota.Results Compared to the control group,gut microbiota richness was signifi-cantly reduced in the cigar-exposed groups(P<0.05).Compared with thecontrol group,the Shannon index of mice in the high-dose group was significantly increased(P<0.05).In multi-group comparisons,ten bacterial genera with high abundance-such as Akkermansia,Allobaculum,and Alloprevotella-were identified.Pairwise comparison results indicated that compared to the control group,abundances of Akkermansia,Candidatus_Sac-charimonas,and Lactobacillus decreased while those of Allobaculum,Alloprevotella,Muribaculaceae,and Pre-votellaceae_UCG001 increased(P<0.05).Alistipes and Faecalibaculum showed significant increases in low-dose and medium-dose groups respectively,Blautia and Lachnospiraceae_NK4A136 group exhibited notable in-creases in the high-dose group(P<0.05).Linear discriminant analysis effect size revealed that six phyla and forty-four species displayed significant differences across all groups at both phylum and species levels,distinct dose-specific were observed among different cigar exposure groups.Conclusion Cigar smoke exposure and different exposure concentrations can both cause changes in the gut microbiota.The effects of different con-centrations of cigars on the gut microbiota of mice are specific.
