Effects of different pretreatment methods on the culture results of Mycobacterium marinus in tissue specimens
10.3969/j.issn.1673-4130.2025.12.001
- VernacularTitle:皮损组织不同前处理方法对海分枝杆菌培养结果的影响
- Author:
Suxiang LIN
1
;
Yun XIANG
;
Sufen YI
;
Wenfang TIAN
;
Xijun TANG
Author Information
1. 珠海市中西医结合医院检验科,广东珠海 519000
- Keywords:
specimen pre-treatment;
Mycobacterium marinum;
culture
- From:
International Journal of Laboratory Medicine
2025;46(12):1409-1413
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of different methods on the culture results of Mycobacterium marinum,including direct inoculation,grinding followed by digestion,and digestion followed by grinding.Methods From June 2022 to March 2024,122 patients with suspected Mycobacterium marinum infection were collected from the Department of Dermatology in this hospital,including 44 cases with superficial skin type and 78 cases with subcutaneous tissue type.Direct inoculation,grinding followed by and digestion followed by grinding were used respectively to analyze the effects of these three methods on the positive detection rate and contamination rate of Mycobacterium marinum culture.Results The culture positive detection rate of Myco-bacterium marinum in superficial skin type and in subcutaneous tissue type were 47.7％(21/44)and 67.9％(53/78),respectively.The use of grinding combined with digestion method for processing skin or subcutane-ous tissue specimens results in a higher positive detection rate and a lower contamination rate compared with the direct inoculation method,with statistically significant differences(P＜0.05).For superficial skin type specimens,the grinding followed by digestion method had a higher positive detection and a lower contamina-tion rate than the digestion followed by grinding method,with statistically significant differences(P＜0.05).For subcutaneous tissue samples,the grinding followed by digestion method had a higher positive detection rate compared to the digestion followed by grinding method,but the difference was not statistically significant(P＞0.05).There were no significant differences in the primary culture time of Mycobacterium marinum a-mong the three pretreatment methods(P＞0.05).Conclusion The pretreatment of skin tissue samples with grinding followed by digestion,and the pretreatment of subcutaneous tissue samples with grinding followed by digestion or digestion followed by grinding can significantly improve the positive rate of Mycobacterium mari-num culture and reduce the contamination rate of miscellaneous bacteria.
