Interpretation of Chinese expert consensus on flow cytometric detection of hematological malignant cells in tissue samples
10.3969/j.issn.1673-4130.2025.11.001
- VernacularTitle:《流式细胞术检测组织样本中血液肿瘤细胞的中国专家共识》解读
- Author:
Liangmei LI
1
;
Shuang CHEN
;
Lian LI
;
Zailin YANG
;
Xia MAO
;
Mingxia ZHU
;
Hongmei JING
;
Min XIAO
;
Yao LIU
;
Yanrong LIU
Author Information
1. 重庆大学附属肿瘤医院血液肿瘤中心/肿瘤转移与个体化诊治转化研究重庆市重点实验室,重庆 400030
- Keywords:
flow cytometry;
tissue samples;
hematologic malignancies;
consensus;
interpretation
- From:
International Journal of Laboratory Medicine
2025;46(11):1281-1289
- CountryChina
- Language:Chinese
-
Abstract:
Hematologic malignancies,such as lymphoma,myeloma,and myeloid neoplasms,can occur in extramedullary tissues.Traditional histopathological morphology and immunohistochemical staining have lim-itations,including time-consuming specimen processing,prolonged reporting cycles,and relatively low sensi-tivity in cases of limited cell numbers.Flow cytometry offers significant advantages in detecting tissue sam-ples,such as rapid processing,shorter reporting cycles,and high accuracy and sensitivity,making it an effective complement to histopathological and immunohistochemical methods.However,the application of flow cytome-try in tissue sample detection currently lacks standardized protocols for sample collection and preservation,single-cell suspension preparation,antibody panel design for limited samples,data analysis,and result repor-ting.To promote the standardized application of flow cytometry in detecting hematologic tumor cells in tissue samples,the Cell Analysis Professional Committee of the Chinese Society of Biotechnology organized experts to develop the Chinese Expert Consensus on Flow Cytometry for Detecting Hematologic Tumor Cells in Tis-sue Samples(hereinafter referred to as the Consensus).This Consensus elaborates on the technical aspects of flow cytometry for tissue sample detection,covering sample processing,antibody panel design,data analysis,reporting content,and quality management.It particularly emphasizes recommended antibody panels and data analysis methods for flow cytometry when tissue sample cell counts are low.This article aims to interpret the key points of the Consensus to facilitate its better application in clinical practice.
