Effect and mechanism of EZH2 on hepatocellular carcinoma cell proliferation by regulating Wnt/β-catenin signaling pathway
10.3969/j.issn.1673-4130.2025.10.006
- VernacularTitle:EZH2通过调控Wnt/β-catenin信号通路对肝癌细胞增殖的影响及作用机制
- Author:
Jian ZHANG
1
;
Liu YANG
;
Yuan FENG
;
Zhihui CHEN
Author Information
1. 南充市中心医院普外一科,四川南充 637000
- Keywords:
enhancer of Zeste homolog 2;
Wnt/β-catenin signaling pathway;
hepatocellular carcinoma HepG2 cells;
cell proliferation;
siRNA
- From:
International Journal of Laboratory Medicine
2025;46(10):1180-1184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of enhancer of Zeste homolog 2(EZH2)on hepatocellular car-cinoma HepG2 cell proliferation by inhibiting Wnt/β-catenin signaling pathway.Methods The expression lev-els of mRNA and protein of EZH2 in hepatocellular carcinoma HepG2,SMMC-7721,BEL-7402 cells and nor-mal hepatocytes were detected by real time fluorescence quantitative reverse transcription polymerase chain reaction and Western blot.Transfected siRNA EZH2 and siRNA control in hepatocellular carcinoma HepG2 cells.siRNA EZH2 in HepG2 cells and siRNA control were transfected,MTT was used to measure the HepG2 cells' ability to proliferate.Western blot technique was used to identify the expression levels of proteins.By transfecting siRNA EZH2 in HepG2 cells and administering an activator of the Wnt/β-catenin signaling path-way,MTT assay was used to determine the proliferation ability of cells.Results The EZH2 mRNA and EZH2 protein levels in hepatocellular carcinoma HepG2,SMMC-7721 and BEL-7402 cells were higher than those in normal hepatocytes(P＜0.05),and the mRNA and protein levels of EZH2 in HepG2 cells were high-er than those in SMMC-7721 and BEL-7402 cells(P＜0.05).siRNA EZH2 could significantly inhibit the mR-NA and protein levels of EZH2 in hepatocellular carcinoma HepG2 cells.After EZH2 expression inhibition,the cell viability of HepG2 cells was lower at 24,48,72 h(P＜0.05),Wnt 1 and β-catenin protein levels were lower(P＜0.05).Conclusion EZH2 is overexpressed in hepatocellular carcinoma HepG2 cells,and knock-down of EZH2 significantly inhibits the proliferative ability of HepG2 cells and decreases the levels of proteins related to the Wnt/β-catenin signaling pathway.
