Relationship between peripheral blood B10 cells and TGF-β expression in children with chronic gastroenteritis and Hp infection and their condition and prognosis
10.3969/j.issn.1673-4130.2025.10.003
- VernacularTitle:慢性肠胃炎伴Hp感染患儿外周血B10细胞比例、TGF-β水平与病情及预后关系
- Author:
Qiuyan DU
1
;
Jing LIU
Author Information
1. 延安大学咸阳医院儿科,陕西咸阳 712000
- Keywords:
chronic gastroenteritis;
Helicobacter pylori;
children;
B10 cells;
transforming growth factor-β;
condition;
prognosis
- From:
International Journal of Laboratory Medicine
2025;46(10):1163-1166,1172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the expression of B10 cells and transforming growth factor-β(TGF-β)in peripheral blood of children with chronic gastroenteritis accompanied by Helico-bacter pylori(Hp)infection and their condition and prognosis.Methods A total of 104 children with chronic gastroenteritis accompanied by Hp infection admitted to Xianyang Hospital,Yan'an University from Decem-ber 2021 to December 2023 were retrospectively selected as the research subjects.According to the visual ana-logue scale(VAS)score,endoscopic examination results and histopathological examination results,the chil-dren were divided into mild group(n=37),moderate group(n=41)and severe group(n=26).All children were treated with standard triple therapy for 14 days.Follow-up consultation was conducted 4 weeks after the end of treatment,and the patient's prognosis was determined based on the results of gastroscopy and urease test.The children were divided into good prognosis group(n=82)and poor prognosis group(n=22).The pe-ripheral blood of the children was collected before treatment and 4 weeks after the end of treatment,and the proportion of B10 cells and the level of TGF-β were measured respectively.The general information,the pro-portion of B10 cells in peripheral blood,and the expression of TGF-β were compared between the mild group and the moderate group,among the three subgroups in the moderate group,and between the poor prognosis group and the good prognosis group.The independent sample t test was used to analyze the differences in quantitative parameter values of each group,Spearman correlation coefficient was used to analyze the correla-tion between the disease condition and the peripheral blood B10 cells and TGF-β level,and the receiver operat-ing characteristic(ROC)curve was used to analyze the predictive value of peripheral blood B10 cell proportion and TGF-β level for poor prognosis in pediatric patients.Results Before treatment,the proportion of B10 cells and TGF-β level in the peripheral blood of children decreased sequentially in severe group,moderate group and mild group,and were positively correlated with the severity of the disease(P＜0.05).After treatment,the proportion of B10 cells and TGF-β level in the peripheral blood of the children were significantly reduced com-pared to before treatment(P＜0.05).4 weeks after the end of treatment,the proportion of B10 cells and TGF-β level in the peripheral blood of children in the poor prognosis group were significantly higher than those in the good prognosis group(P＜0.05).After treatment,the proportion of B10 cells and the level of TGF-β in children's peripheral blood had certain predictive value for the prognosis of children.The area under the curve of ROC curve analysis was 0.759 and 0.743,respectively,which was lower than 0.886 of combined prediction(Z=-2.445,2.391,P=0.014,0.017),and the sensitivity and specificity of combined prediction were 77.34％and 91.45％respectively.Conclusion The proportion of B10 cells and TGF-β level before treatment are positively correlated with the severity of the disease,and the combined detection of the two after treatment has good predictive value for the prognosis of children.
