Relationship between serum miRNA-132 and miRNA-345 expression and tumor markers and prognosis in gastric cancer patients
10.3969/j.issn.1673-4130.2025.09.016
- VernacularTitle:胃癌患者血清miRNA-132和miRNA-345表达与肿瘤标志物及预后关系研究
- Author:
Chong ZHU
1
;
Kefeng GUO
;
Yun ZHAO
;
Dan HUANG
Author Information
1. 黄河三门峡医院:医学检验科,河南三门峡 472000
- Keywords:
gastric cancer;
microRNA-132;
microRNA-345;
tumor markers;
prognosis
- From:
International Journal of Laboratory Medicine
2025;46(9):1104-1107,1113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between serum microRNA-132(miRNA-132)and mi-croRNA-345(miRNA-345)expression,tumor markers and prognosis in gastric cancer patients.Methods A total of 103 gastric cancer patients from the hospital from January 2020 to January 2022 were selected as study group.82 patients with benign gastric diseases in the hospital during the same period were selected as the benign group.Another 61 healthy individuals who underwent physical examinations in the hospital during the same period were selected as control group.Real-time polymerase chain reaction(qPCR)was used to measure the expression of miRNA-132 and miRNA-345.The levels of carcinoembryonic antigen(CEA),carbohydrate anti-gen 199(CA199)and glycoprotein antigen 724(CA724)were measured using electrochemiluminescence.All patients were followed up until June 2024,and the overall survival(OS)of gastric cancer patients was calcu-lated through outpatient or telephone follow-up.Results The relative expression level of miRNA-132 in the serum of the study group was higher than that of the benign group and the control group(P＜0.05),while the relative expression level of miRNA-345 was lower than that of the benign group and the control group(P＜0.05).The serum levels of CEA,CA199,and CA724 in the study group were higher than those in the be-nign group and the control group(P＜0.05).Pearson analysis showed that miRNA-132 was positively correla-ted with CEA,CA199,and CA724(P＜0.05),while miRNA-345 was negatively correlated with CEA,CA199,and CA724(P＜0.05).Patients with a relative expression level of miRNA-132≤2.76 had better OS than those with a relative expression level of miRNA-132＞2.76(P＜0.05).Patients with a relative expres-sion level of miRNA-132 greater than 0.37 have better OS than those with a relative expression level of miR-NA-132≤0.37(P＜0.05).High expression of miRNA-132 and low expression of miRNA-345 were risk fac-tors affecting the prognosis of gastric cancer patients(P＜0.05).Conclusion Patients with gastric cancer have high expression of miRNA-132 and low expression of miRNA-345,and the expression of miRNA-132 and miRNA-345 is closely related to tumor markers and prognosis,which is worthy of clinical reference.
