Improvement effect of rhodopsin on the hepatic function and intestinal mucosal barrier damage in cirrhotic rats
10.3969/j.issn.1673-4130.2025.08.012
- VernacularTitle:大黄素对肝硬化大鼠肝功能及肠黏膜屏障损伤的改善作用
- Author:
Qiyang MA
1
;
Gaozhan PAN
Author Information
1. 西安国际医学中心医院:感染肝病科,陕西西安 710100
- Keywords:
rhodopsin;
cirrhosis;
intestinal mucosal barrier;
Toll like receptor 4/nuclear factor kap-pa B pathway;
inflammatory response
- From:
International Journal of Laboratory Medicine
2025;46(8):960-964
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the improvement effect of rhodopsin on the hepatic function and intes-tinal mucosal barrier damage in cirrhotic rats by regulating the expression of inflammatory factors.Methods Thir-ty-six male rats were randomly divided into normal group,model group and rhodopsin group,12 rats in each group.Except for the normal group,the rats in the remaining groups were used to induce rat cirrhosis model by carbon tetrachloride(CCl4)-corn oil twice/week for a total of 12 weeks.After successful modeling,rhodop-sin(rhodopsin group)and saline(model group)interventions were given once daily for 4 weeks,respectively.The pathological and structural changes in rat liver and intestinal tissues were observed by HE and Sirus Red staining.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),alpha hydroxypropionic acid(α-LA),diamine oxidase(DAO),and plasma endotoxin(ET)and inter-leukin(IL)-6,tumor necrosis factor alpha(TNF-α),and IL-17 in ileal tissues were detected.Western blot was used to detect the expression levels of Toll like receptor 4(TLR4)and nuclear factor kappa B(NF-κB)pro-teins in intestinal tissue.Results Compared with normal group,the model group showed pathological changes such as hepatocyte necrosis,disordered arrangement,shedding of intestinal mucosal villi,infiltration of inflam-matory factors and so on.Compared with the model group,inflammatory factors and the degree of injury in liver and intestinal tissue of rats in the rhodopsin group were improved,and the serum levels of AST,ALT,TBIL,IL-6,TNF-α,IL-17,α-LA,DAO,ET levels and TLR4 and NF-κP protein expression levels were signifi-cantly reduced(P＜0.05).Conclusion Rhodopsin is able to attenuate the inflammatory response by inhibi-ting the release of inflammatory factors,attenuate the inflammatory response thereby improving liver func-tion,decreasing α-LA,DAO,and ET levels,and alleviating intestinal mucosal barrier damage.
