Significance of high-throughput sequencing combined with lactate dehydrogenase in predicting severe Mycoplasma pneumonia pneumonia in children
10.3969/j.issn.1673-4130.2025.06.013
- VernacularTitle:高通量测序联合乳酸脱氢酶预测儿童重症肺炎支原体肺炎的意义
- Author:
Wenzheng WANG
1
;
Xiulan LAI
;
Guiqiu LI
;
Hui GONG
;
Jianxing LAO
;
Ying WEI
Author Information
1. 佳木斯大学临床医学院,黑龙江 佳木斯 154003
- Keywords:
severe Mycoplasma pneumoniae pneumonia;
high-throughput sequencing;
lactate dehydro-genase;
drug resistance genes
- From:
International Journal of Laboratory Medicine
2025;46(6):709-713
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the predictive value of serum lactate dehydrogenase(LDH)level com-bined with high-throughput sequencing in children with severe Mycoplasma pneumoniae pneumonia(SMPP).Methods The clinical data of 99 children with Mycoplasma pneumoniae pneumonia(MPP)admitted to Hua-zhong University of Science and Technology Union Hospital from October 2023 to March 2024 were retro-spectively analyzed.According to the severity of the disease,the children were divided into mild group(33 ca-ses)and severe group(66 cases).Spearman correlation analysis was used for correlation analysis,Logistic re-gression was used to analyze the influencing factors of SMPP,and the receiver operating characteristic(ROC)curve was used to evaluate the value of LDH in predicting SMPP.Results The level of LDH in the severe group was higher than that in the mild group(P<0.05).The results of high-throughput sequencing showed that the proportions of virulence positive and drug resistance gene A2063G mutation in the severe group were higher than those in the mild group(P<0.05).Multivariate Logistic regression analysis showed that LDH level and drug resistance gene A2063G mutation were related to the SMPP(P<0.05).ROC curve analysis showed that LDH combined with high-throughput sequencing detection of drug resistance gene A2063G muta-tion had a high value in predicting SMPP,and the area under the curve was 0.724.Conclusion Serum LDH combined with high-throughput sequencing detection of drug resistance gene A2063G mutation can be used as an effective indicator to predict SMPP.
