The levels of NK cells and Th cytokines predict the outcomes of assisted reproductive pregnancy in HR-HPV infected patients
10.3969/j.issn.1673-4130.2025.04.004
- VernacularTitle:NK细胞及Th细胞因子水平预测HR-HPV感染者辅助生殖妊娠结局
- Author:
Hailing WEN
1
;
Haohui LI
;
Lihua ZHOU
Author Information
1. 海南医学院第二附属医院生殖医学科,海南海口 570311
- Keywords:
natural killer cells;
high risk human papillomavirus;
assisted reproduction
- From:
International Journal of Laboratory Medicine
2025;46(4):404-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the prediction of natural killer(NK)cells,and T helper cells(Th)cy-tokine levels for assisted reproductive pregnancy outcomes in high-risk human papillomavirus(HR-HPV)in-fected patients.Methods A total of 128 cases of HR-HPV infected patients who received assisted reproduc-tive treatment in a hospital from September 2021 to June 2023 were selected as the study objects,and were di-vided into normal pregnancy outcome group(n=87)and adverse pregnancy outcome group(n=41)accord-ing to their pregnancy outcomes.The clinical data,NK cell proportion,interleukin(IL)-2,IL-4,IL-6 and IL-10 levels before in vitro fertilization-embryo transfer treatment were collected and compared between the two groups.Spearman correlation analysis,univariate Logistic regression analysis and multiple Logistic regression analysis were used to screen out the risk factors for adverse pregnancy outcomes in HR-HPV infected patients receiving assisted reproduction.Receiver operating characteristic(ROC)curve and area under the curve(AUC)were used to evaluate the predictive efficacy of risk factors alone and in combination for adverse preg-nancy outcomes in HR-HPV infected patients undergoing assisted reproduction.Results The age of the ad-verse pregnancy outcome group was significantly higher than that of the normal pregnancy outcome group,and the difference was statistically significant(P＜0.05).The proportion of NK cells and the level of IL-10 in the adverse pregnancy outcome group were significantly lower than those in the normal pregnancy outcome group,and the level of IL-6 was significantly higher than that in the normal pregnancy outcome group,with statistical significance(P＜0.05).Spearman correlation analysis showed that age and IL-6 level of HR-HPV infection were positively correlated with adverse pregnancy outcome after assisted reproduction(r=0.582,0.341,P＜0.001).The proportion of NK cells and the level of IL-10 were negatively correlated with adverse pregnancy outcomes after assisted reproduction(r=-0.459,-0.359,P＜0.001).Multivariate Logistic re-gression analysis showed that the proportion of NK cells,low level of IL-10 and high level of IL-6 in HR-HPV infected persons were risk factors for adverse pregnancy outcomes after assisted reproduction(P＜0.05).ROC curve analysis results showed that the AUC of NK cell ratio,IL-6 and IL-10 alone and in combination to predict adverse pregnancy outcome after assisted reproduction in HR-HPV infected patients were 0.784,0.711,0.660 and 0.876,respectively.The efficiency of combined prediction using the three indicators was higher than that of independent prediction(Z=3.006,3.211,3.716,P＜0.05).Conclusion The levels of NK cells and Th cytokines have important clinical significance in the prediction of assisted reproductive pregnancy outcome in HR-HPV infected patients.
