Relationship between COL1A1,MUC4 expression and clinicopathological features and prognosis in patients with nasopharyngeal carcinoma
10.3969/j.issn.1673-4130.2025.04.002
- VernacularTitle:鼻咽癌患者COL1A1、MUC4表达与临床病理特征及预后的关系
- Author:
Wei ZHOU
1
;
Hanlin LI
Author Information
1. 江汉大学附属医院武汉市第六医院耳鼻喉科,湖北武汉 430015
- Keywords:
nasopharyngeal carcinoma;
collagen type Ⅰ alpha 1 chain;
mucin 4;
prognosis
- From:
International Journal of Laboratory Medicine
2025;46(4):391-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between collagen type Ⅰ alpha 1 chain(COL1A1)and mucin 4(MUC4)expression and clinicopathological features and prognosis in patients with nasopharynge-al carcinoma.Methods A total of 269 suspected patients with nasopharyngeal carcinoma admitted to a hospi-tal from December 2020 to June 2021 were selected as the study subjects,123 patients with nasopharyngeal carcinoma confirmed by pathological examination were selected as the study group,and 146 patients with na-sopharyngeal carcinoma ruled out by pathological examination were selected as the control group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the mRNA expression of COL1A1 and MUC4,and immunohistochemical staining was used to detect the protein expression of COL1A1 and MUC4.Survival curves were drawn by Kaplan-Meier method to analyze the relationship between COL1A1 and MUC4 protein expression and survival of nasopharyngeal carcinoma patients.COX regression analysis of nasopharyn-geal carcinoma patients with adverse prognostic factors.Receiver operating characteristic(ROC)curve was drawn to analyze the value of COL1A1 and MUC4 mRNA expression in predicting poor prognosis of patients with nasopharyngeal carcinoma.Results Compared with the control group,the mRNA expressions of COL1A1 and MUC4 in the study group were increased,and the difference was statistically significant(P＜0.05).The positive expression rate of COL1A1 protein in the study group was 58.54％(72/123)higher than that in the control group[41.10％(60/146)],and the difference was statistically significant(P＜0.05).The positive expression rate of MUC4 protein was 63.41％(78/123)higher than that of control group[33.56％(49/146)],and the difference was statistically significant(P＜0.05).The expressions of COL1A1 and MUC4 protein were correlated with TNM stage,depth of tumor invasion and lymph node metastasis(P＜0.05).Compared with the patients with good prognosis,the expressions of COL1A1 and MUC4 mRNA were in-creased in the patients with poor prognosis,and the differences were statistically significant(P＜0.05).The 3-year cumulative survival rate of patients with positive expression of COL1A1 protein was significantly lower than that of patients with negative expression of COL1A1 protein,and the difference was statistically signifi-cant(P＜0.05).The 3-year cumulative survival rate of patients with positive expression of MUC4 protein was significantly lower than that of patients with negative expression of MUC4 protein,and the difference was sta-tistically significant(P＜0.05).TNM stage Ⅲ/Ⅳ,tumor invasion stage T3/T4,lymph node metastasis,posi-tive expression of COL1A1 and MUC4 protein were risk factors for poor prognosis(P＜0.05).ROC curve a-nalysis results showed that the area under the curve(AUC)of COL1A1 and MUC4 mRNA combined to pre-dict poor prognosis of nasopharyngeal carcinoma patients was significantly greater than the AUC of MUC4 mRNA alone,and the difference was statistically significant(Z=2.248,P=0.025).Conclusion The expres-sion of COL1A1 and MUC4 in patients with nasopharyngeal carcinoma is increased,which is closely related to the clinicopathological features and prognosis of patients with nasopharyngeal carcinoma,and has important reference value for the prognosis evaluation of patients with nasopharyngeal carcinoma.
