Western blot band patterns analysis of 1 347 HIV-1 antibody positive samples
10.3969/j.issn.1673-4130.2025.03.014
- VernacularTitle:1347例HIV-1抗体阳性标本蛋白质印迹带型分析
- Author:
Jiao YU
1
;
Min SONG
Author Information
1. 中国人民解放军陆军军医大学第一附属医院输血科,重庆 400030
- Keywords:
human immunodeficiency virus;
Western blot;
band patterns
- From:
International Journal of Laboratory Medicine
2025;46(3):325-328,334
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the Western blot(WB)band patterns of 1 347 human immunodeficiency virus(HIV)-1 antibody positive specimens.Methods A total of 1 347 HIV-1 antibody positive specimens confirmed by WB method were collected in a hospital from 2013 to 2023,and WB band patterns distribution of HIV-1 antibody positive specimens was compared according to gender,age,infection route and viral load.Re-sults Among 1 347 HIV-1 antibody positive specimens,the WB band patterns were dominated by 7 bands(22.6%),8 bands(20.2%),9 bands(14.8%)and all bands(24.4%).The positive rate of p55 band in female HIV-1 antibody positive specimens was significantly higher than that in male HIV-1 antibody positive speci-mens,and the difference was statistically significant(P<0.05).Compared with other age groups,the positive rate of p31 band in HIV-1 antibody positive specimens aged 18-<40 years was the highest(84.0%),and the difference was statistically significant(P<0.05).The positive rates of p31 and gp41 band in HIV-1 antibody positive specimens of men with gay sex were significantly lower than those in HIV-1 antibody positive speci-mens of heterosexual sex,and the difference was statistically significant(P<0.05).There was no significant difference in the positive rate of WB band pattern among HIV-1 antibody positive specimens with different vi-ral loads(P>0.05).Conclusion Gp160,gp120,gp41 and p24 are essential bands to confirm HIV-1 antibody positive,and have no obvious relationship with region,genetic background,HIV-1 subtype,etc.In addition,other bands have some differences in different regions of the population,according to these differences,accu-rate prevention and treatment of acquired immunodeficiency syndrome population can be carried out.
