Value of bronchoalveolar lavage fluid metagenomic next-generation sequencing in pathogen diagnosis of pulmonary infection in children
10.3969/j.issn.1673-4130.2025.02.006
- VernacularTitle:支气管肺泡灌洗液宏基因组二代测序在儿童肺部感染病原体诊断中的价值
- Author:
Jingjing WANG
1
;
Chong ZHANG
Author Information
1. 甘肃中医药大学公共卫生学院,甘肃兰州 730050;甘肃省妇幼保健院科研中心,甘肃兰州 730050
- Keywords:
metagenomic next-generation sequencing;
conventional microbiology testing;
bronchoalve-olar lavage fluid;
pulmonary infection
- From:
International Journal of Laboratory Medicine
2025;46(2):157-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnostic value of bronchoalveolar lavage fluid(BALF)met-agenomic next-generation sequencing(mNGS)in pulmonary infection in children.Methods The clinical data of 109 children with pulmonary infection who were hospitalized in Gansu Provincial Maternal and Child Health Hospital from March 1,2023 to March 1,2024 were analyzed.BALF was collected for the etiological detection of mNGS,and the results were compared with those of conventional microbiology testing(CMT).Results The detection rate of BALF mNGS in 109 cases(96.3％)was higher than that of CMT(66.1％),and the difference was statistically significant(P＜0.01).A total of 280 strains of pathogens were detected in BALF mNGS and 130 strains were detected in CMT.Compared with CMT,mNGS could detect more patho-gens,especially the detection rate of viruses is much higher than that of CMT.Among the 70 mNGS and CMT double-positive samples,42 cases were completely or partially matched with the results of mNGS and CMT,48％of the matched samples were Haemophilus influenzae,19％were Mycoplasma pneumoniae,and 17％were Streptococcus pneumoniae.The pathogenic bacteria characteristics of BALF mNGS in children with se-vere pneumonia were analyzed.The detection rates of Haemophilus influenzae,Parainfluenza virus and Human metapneumovirus in children with severe pneumonia were higher than those in children without severe pneu-monia,and the differences were statistically significant(P＜0.05).In the comparative analysis of BALF mNGS detection results between full-term normal weight children and premature low weight children with birth information,it was found that the detection rate of Pseudomonas aeruginosa mNGS in premature low weight children was higher than that in full-term normal weight children,and the detection rate of Pseudo-monas aeruginosa mNGS in premature low weight children with complications was higher than that in prema-ture low weight children without complications,and the differences were statistically significant(P＜0.05).The detection rate of Mycoplasma pneumoniae mNGS in full-term normal weight children was higher than that in preterm low weight children,and the detection rate of Mycoplasma pneumoniae mNGS in full-term normal weight children without complications was higher than that in full-term normal weight children with complications,and the differences were statistically significant(P＜0.05).Conclusion BALF mNGS has a higher microbiological assay in children with pulmonary infection positive rate of detection,especially for severe infec-tions and complex cases,and BALF mNGS is an important supplement to routine etiological detection.
