Repair mechanism of Echinetin on intestinal mucosal barrier damage in severe acute pancreatitis based on HMGB1/TLR4/PKR pathway
10.3969/j.issn.1673-4130.2025.02.004
- VernacularTitle:基于HMGB1/TLR4/PKR通路探讨松果菊苷对重症急性胰腺炎肠道黏膜屏障损伤的修复机制
- Author:
Manhe ZHANG
1
;
Kejing YU
;
Shihao JIA
;
Fumei ZHANG
;
Jingcha HAO
Author Information
1. 河北省沧州中西医结合医院急诊科,河北沧州 061001
- Keywords:
Echinoside;
HMGB1/TLR4/PKR pathway;
acute pancreatitis;
intestinal mucosal
- From:
International Journal of Laboratory Medicine
2025;46(2):146-150,156
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of Echinetin(ECH)pretreatment on alleviating intestinal bar-rier dysfunction caused by severe acute pancreatitis(SAP).Methods Totally 36 rats were randomly divided into Sham group,SAP group and SAP+ECH group,with 12 rats in each group.Pancreatitis was induced by retrograde injection of 3％sodium taurocholate into pancreatic duct.Histological examination was performed on the pancreas of experimental rats to determine whether the pancreatitis model of rats was successfully con-structed.Intestinal barrier function was evaluated by intestinal pathological scores,serum diamine oxidase(DAO)activity and endotoxin levels,and bacterial translocation in mesenteric lymph nodes.The mRNA and protein expression levels of tight junction proteins ZO-1 and occludin were detected by quantitative fluorescent PCR(qPCR)and Western blot,and the protein expression levels of high mobility frame-1 protein(HMGB1),Toll-like receptor 4(TLR4)and protein kinase R(PKR)were detected by Western blot.Results ECH had no significant effect on the histological changes of pancreas,but could improve the intestinal mucosal barrier damage and membrane permeability associated with SAP.Although ECH does not affect the mRNA expres-sion levels of ZO-1 and occludin in ileum of SAP rats,it could significantly increase the expression levels of ZO-1 and occludin,and ECH treatment could significantly reduce the expression levels of HMGB1,TLR4 and PKR in ileum of SAP rats.Conclusion ECH can reduce the intestinal barrier dysfunction induced by SAP,and its effect on intestinal barrier function may be related to the inhibition of the HMGB1/TLR4/PKR pathway.
