LINC01667 reprogram fatty acid metabolism in hepatocellular carcinoma by up-regulation of FABP5
10.3969/j.issn.1673-4130.2025.02.001
- VernacularTitle:LINC01667通过上调FABP5重塑肝细胞癌脂肪酸代谢
- Author:
Kainan ZHANG
1
;
Xiang LI
;
Jianfeng FU
Author Information
1. 新疆医科大学研究生学院,新疆乌鲁木齐 830017;新疆维吾尔自治区人民医院检验科,新疆乌鲁木齐 830000
- Keywords:
hepatocellular carcinoma;
LINC01667;
fatty acid binding protein 5;
fatty acid metabolism
- From:
International Journal of Laboratory Medicine
2025;46(2):129-134,140
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulation of LINC01667 on fatty acid binding protein 5(FABP5)and its effect on fatty acid metabolism in hepatocellular carcinoma(HCC)cells.Methods Based on the dual luciferase reporter assay,the binding ability of LINC01667 to FABP5 promoter and its regulatory effect on promoter activity were detected..In addition,the effects of overexpression of LINC01667 on the content of in-tracellular lipid droplets in HCC cells and on the cellular free fatty acid uptake capacity were detected by Nile red staining and free fatty acid uptake assay,respectively.The effect of LINC01667/FABP5 on the migration and invasion of HCC cells was observed by Transwell experiment.Results LINC01667 could bind to FABP5 promoter and up-regulate FABP5 expression(P＜0.05).Overexpression of LINC01667 could enhance the ac-cumulation of lipid droplets in HepG2 and HuH7 cells and enhance the uptake of free fatty acids(P＜0.05).After targeted inhibition of FABP5,the enhancement of free fatty acid uptake of HCC cells mediated by LINC01667 and its migration and invasion ability were significantly weakened(P＜0.01).Conclusion LINC01667 could remodel the fatty acid metabolism of HCC cells through the up-regulation of FABP5,and then promote the malignant process of HCC.
