Expression and clinical significance of MIP-3a,IL-37 and CCL5 in serum of patients with colorectal cancer
10.3969/j.issn.1673-4130.2024.24.014
- VernacularTitle:结直肠癌患者血清MIP-3α、IL-37、CCL5表达及临床意义
- Author:
Xinwei ZHANG
1
;
Tianyu LIANG
Author Information
1. 辽宁省肿瘤医院(中国医科大学肿瘤医院)中西医结合科,辽宁沈阳 110000
- Keywords:
colorectal cancer;
recurrence;
macrophage inflammatory protein-3α;
interleukin-37;
CC chemokine ligand 5
- From:
International Journal of Laboratory Medicine
2024;45(24):3015-3020
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the levels and clinical significance of macrophage inflammatory protein-3a(MIP-3a),interleukin-37(IL-37)and CC chemokine ligand 5(CCL5)in serum macrophages of patients with colorectal cancer.Methods A total of 158 patients with colorectal cancer admitted to Liaoning Cancer Hospi-tal from January 2018 to December 2020 were prospectively selected as the cancer group,and were divided into the recurrence group(41 cases)and the non recurrence group(117 cases)according to the the follow-up situa-tion.158 healthy volunteers who underwent physical examination in Liaoning Cancer Hospital during the same period were selected as control group.Enzyme linked immunosorbent assay(ELISA)was applied to detect se-rum levels of MIP-3α,IL-37 and CCL5.Pearson correlation was applied to analyze the correlation between ser-um levels of MIP-3α,IL-3 and CCL5 in cancer group.Multivariate Logistic regression was applied to analyze the factors affecting postoperative recurrence in colorectal cancer patients.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum MIP-3α,IL-37 and CCL5 in combination for postoperative recurrence in colorectal cancer patients.Results The serum levels of MIP-3α and CCL5 in the cancer group were obviously higher than those in the control group,while the level of IL-37 was obviously lower than that in the control group(P＜0.05).Compared with the non recurrence group,the proportion of patients with TNM stage Ⅲ in the recurrence group was higher(P＜0.05),and the serum levels of MIP-3αand CCL5 in the recurrence group were obviously increased,while the level of IL-37 was obviously reduced(P＜0.05).Serum MIP-3α was negatively correlated with IL-37 level(r=-0.540,P＜0.05)and was posi-tively correlated with CCL5 level(r=0.623,P＜0.05),and IL-37 was negatively correlated with CCL5 level(r=-0.551,P＜0.05).TNM stage Ⅲ,increased level of MIP-3α and increased level of CCL5 were risk fac-tors for postoperative recurrence in colorectal cancer patients(P＜0.05),while increased level of IL-37 was a protective factor for postoperative recurrence in colorectal cancer patients(P＜0.05).The area under the curve(AUC)of the combination of serum MIP-3α,IL-37 and CCL5 in predicting postoperative recurrence in colorectal cancer patients was 0.889(95％CI:0.830-0.948),the AUC of MIP-3α,IL-37,and CCL5 alone in predicting postoperative recurrence in colorectal cancer patients was 0.780(95％CI:0.692-0.868),0.755(95％CI:0.673-0.838),and 0.803(95％CI:0.730-0.876),respectively.AUC of the combination of the three was higher than that of MIP-3α(Z=0.015,P=0.022),IL-37(Z=2.596,P=0.005),and CCL5(Z=1.805,P=0.036)alone.The relapse-free survival rate of patients with high MIP-3α expression(55/86,63.95％)was lower than that of patients with low MIP-3α expression(62/72,86.11％,Log-Rank x2=11.709,P＜0.05).The relapse-free survival rate of patients with high IL-37 expression(77/89,86.62％)was higher than that of patients with low IL-37 expression(40/69,57.97％,Log-Rank x2=19.917,P＜0.05).The relapse-free survival in patients with high CCL5 expression(51/84,60.71％)was lower than that in pa-tients with low CCL5 expression(66/74,89.19％,Log-Rank x2=19.366,P＜0.05).Conclusion The high expression of MIP-3α and CCL5 and low expression of IL-37 in the serum of patients with postoperative recur-rence of colorectal cancer are all related to the postoperative relapse-free survival rate of patients,and the com-bination of the three could be used to predict postoperative recurrence of colorectal cancer patients,and the clinical application value is relatively high.
