Changes in serum lncRNA GAS5 and TIMP3 levels in children with mycoplasma pneumoniae pneumonia and their clinical significance
10.3969/j.issn.1673-4130.2024.24.008
- VernacularTitle:肺炎支原体肺炎患儿血清lncRNA GAS5、TIMP3水平变化及临床意义
- Author:
Caiyun HAN
1
;
Jiao MA
;
Xiaohui LI
Author Information
1. 榆林市星元医院儿科,陕西榆林 719000
- Keywords:
mycoplasma pneumoniae pneumonia;
long noncoding ribonucleic acid growth arrest specific 5;
tissue inhibitor of matrix metalloproteinases 3;
severity of disease;
prognosis
- From:
International Journal of Laboratory Medicine
2024;45(24):2983-2988
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in the levels of serum long noncoding ribonucleic acid(ln-cRNA)growth arrest specific 5(GAS5)and tissue inhibitor of matrix metalloproteinases(TIMP)3 and the clinical significance in children with mycoplasma pneumoniae pneumonia(MPP).Methods A total of 150 children with MPP admitted to Department of Pediatrics of Yulin Xingyuan Hospital from January 2021 to October 2023 were selected as MPP group and were divided into a severe group(98 cases)and a mild group(52 cases)according to the severity of disease,and were divided into a poor prognosis group(31 cases)and a good prognosis group(119 cases)according to the 28 d prognosis.Another 60 healthy children during the same period were selected as the control group.Serum lncRNA GAS5 and TIMP3 levels were measured by re-al-time fluorescence quantitative polymerase chain reaction and enzyme-linked immunosorbent assay respec-tively.Pearson correlation was used to analyze the correlation of serum lncRNA GAS5 and TIMP3 levels in children with MPP,multivariate Logistic regression was used to analyze the factors affecting the poor progno-sis in children with MPP,and receiver operating characteristic curve was used to analyze the predictive value of serum lncRNA GAS5 and TIMP3 levels for poor prognosis in children with MPP.Results Serum lncRNA GAS5 and TIMP3 levels in the severe group were lower than those in the mild group and the control group(P＜0.05),and serum lncRNA GAS5 and TIMP3 levels in the mild group were lower than those in the con-trol group(P＜0.05).Serum lncRNA GAS5 and TIMP3 levels were positively correlated in children with MPP(r=0.633,P＜0.001).The incidence of 28 d poor prognosis in 150 children with MPP was 20.67％(31/150).The proportion of severe cases,peak body temperature,procalcitonin,and C-reactive protein levels in the poor prognosis group were higher than those in the good prognosis group(P＜0.05),the length of hos-pital stay was longer than that in the good prognosis group(P＜0.05),and the levels of lncRNA GAS5 and TIMP3 were lower than those in the good prognosis group(P＜0.05).Critical illness and elevated procalcito-nin level were independent risk factors for poor prognosis in children with MPP,and elevated lncRNA GAS5 and TIMP3 levels were independent protective factors(P＜0.05).The area under the curve of serum lncRNA GAS5 combined with TIMP3 levels for predicting poor prognosis in children with MPP was 0.878,which was greater than 0.780 and 0.778 predicted by serum lncRNA GAS5 and TIMP3 levels alone(P＜0.05).Conclu-sion The decrease in serum lncRNA GAS5 and TIMP3 levels in children with MPP is closely related to the worsening of the condition and poor prognosis,the combination of serum lncRNA GAS5 and TIMP3 has high predictive value for poor prognosis in children with MPP.
