Evaluation of the application value of BALF-EGFR gene mutation in targeted therapy for lung cancer patients based on droplet digital PCR
10.3969/j.issn.1673-4130.2024.23.020
- VernacularTitle:基于微滴式数字PCR的肺癌患者BALF-EGFR基因突变分析在靶向治疗中的应用价值评价
- Author:
Xiaohui LIANG
1
;
Fei WU
;
Wanxing XU
;
Lihua QIAO
Author Information
1. 上海市第一人民医院/上海交通大学医学院附属第一人民医院检验科,上海 200080;上海交通大学医学院医学技术学院,上海 200025
- Keywords:
bronchoalveolar lavage fluid;
droplet digital polymerase chain reaction;
epidermal growth factor receptor;
gene mutation
- From:
International Journal of Laboratory Medicine
2024;45(23):2918-2921
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical application value of bronchoalveolar lavage fluid(BALF)as a specimen for epidermal growth factor receptor(EGFR)gene mutation detection.Methods Droplet digital polymerase chain reaction(ddPCR)was used to detect the mutation situation of relevant sites(L858R and E746_A750del)in EGFR gene in BALF of totally 52 lung cancer patients,and the corresponding molecular pathological results were compared.Results With molecular pathological as a gold standard,the sensitivity of BALF for the detection of E746_A750del was 58.3％(14/24),specificity was 100.0％(14/14)and concord-ance was 73.7％(28/38,Kappa=0.508,P＜0.01),while the sensitivity of BALF for the detection of L858R was 64.3％(9/14),specificity was 100.0％(14/14)and concordance was 82.1％(23/28,Kappa=0.643,P＜0.01).Compared with the molecular pathological results,it showed high concordance in determining mu-tations of E746_A750del and L858R in EGFR gene in BALF(0.40＜Kappa＜0.75).The detection rate of EGFR gene mutation in BALF was related with tumor stage(P＜0.05).The abundance of mutations could predict to some extent the distant metastasis of lung cancer.Conclusion BALF specimens have important clinical application value in detecting EGFR gene mutations in lung cancer patients and could serve as a sup-plement to molecular pathology detection.
