Simultaneous determination of reduced and oxidized coenzyme Q10 in human plasma by liquid chromatography tandem mass spectrometry
10.3969/j.issn.1673-4130.2024.23.018
- VernacularTitle:液相色谱-串联质谱法同时测定人血浆中的还原型和氧化型辅酶Q10
- Author:
Bo YUAN
1
;
Xuan LI
;
Pengfei LI
Author Information
1. 天津市医疗器械质量监督检验中心,天津 300384
- Keywords:
coenzyme Q10;
liquid chromatography-tandem mass spectrometry;
reduced coenzyme Q10;
oxidized coenzyme Q10;
blood drug concentration monitoring
- From:
International Journal of Laboratory Medicine
2024;45(23):2908-2912
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid and sensitive liquid chromatography tandem mass spectrometry(LC-MS/MS)method for the simultaneous determination of reduced coenzyme Q10(ubiquinol-10)and oxi-dized coenzyme Q10(ubiquinone-10)in human plasma,and to achieve clinical application.Methods Plasma samples were precipitated with methanol and protein,and using coenzyme Q9 as the internal standard,Kinetex C18(3.0×50 mm,2.6 μm,100?)column were used for separation with equal elution.Mass spectrometry quantitative detection was carried out by multi reaction monitoring with positive ionization of electrospray ion source.The specificity,standard curve and lower limit of quantification,precision and accuracy,stability of this method were examined.Results The retention times for ubiquinol-10 and ubiquinone-10 were 1.07 and 1.81 minutes,respectively,and the analysis time for a single sample was 3.2 minutes.Ubiquinol-10 and ubiquinone-10 had good linear relationships in the linear range of 50-2 000 and 1-100 ng/mL,respectively,and the low-er limit of quantification could both reach 1 ng/mL.The precision within and between batches were≤9.7％,and the accuracy was between 96.2％-103.9％.This method had high accuracy and precision.Storing at room temperature for 6 hours,refrigerating at 4-8 ℃ for 5 days,and freezing at-20 ℃ for 30 days did not affect the accurate determination of analyte concentration.Conclusion This method has a short analysis time,high sensitivity,and low plasma dosage,and is suitable for monitoring and accurately determining the blood drug concentrations of ubiquinol-10 and ubiquinone-10 in plasma samples.
