Relationship between serum levels of CHI3L1 and HIF-1α and the occurrence of liver fibrosis in patients with chronic hepatitis B complicated with liver cirrhosis
10.3969/j.issn.1673-4130.2024.23.017
- VernacularTitle:血清CHI3L1、HIF-1α水平与慢性乙型肝炎合并肝硬化患者发生肝纤维化的关系
- Author:
Yu ZHENG
1
;
Dan DU
;
Ling LIN
;
Xifeng LEI
Author Information
1. 渭南市中心血站检验科,陕西渭南 714000
- Keywords:
chitinase-3-like protein 1;
hypoxia inducible factor-1α;
chronic hepatitis B;
liver cirrho-sis;
hepatic fibrosis
- From:
International Journal of Laboratory Medicine
2024;45(23):2903-2907
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between serum levels of chitinase-3-like protein 1(CHI3L1)and hypoxia inducible factor-1α(HIF-1α)with the occurrence of liver fibrosis in patients with chronic hepatitis B(CHB)complicated with liver cirrhosis.Methods A total of 90 patients with CHB compli-cated with liver cirrhosis who were treated in Weinan Central Blood Station from March 2021 to September 2023 were selected as the liver cirrhosis group.Additionally,90 patients with CHB during the same period were regarded as the CHB group.According to whether patients with CHB complicated with liver cirrhosis had developed liver fibrosis,they were separated into a non fibrosis group(42 cases)and a fibrosis group(48 cases).Enzyme-linked immunosorbent assay was applied to detect serum levels of interleukin-6(IL-6),high-sensitivity C-reactive protein(hs-CRP),procalcitonin(PCT),CHI3L1,and HIF-1α.Fully automated biochem-ical analyzer was applied to detect levels of glutamate aminotransferase(ALT),aspartate aminotransferase(AST),and alkaline phosphatase(ALP),radioimmunoassay was applied to detect levels of hyaluronic acid(HA),laminin(LN),type Ⅳ collagen(Ⅳ-C),and type Ⅲ procollagen(PC Ⅲ).Pearson correlation was ap-plied to analyze the correlation between CHI3L1,HIF-1α levels and inflammation and liver fibrosis indicators.Receiver operating characteristic curve was applied to analyze the diagnostic value of CHI3L1 and HIF-1α for liver fibrosis in patients with CHB combined with liver cirrhosis.Z-test was applied to compare the differences in the area under the curve(AUC).Results The levels of CHI3L1 and HIF-1α in the liver cirrhosis group were higher than those in the CHB group(P＜0.05),while the levels of IL-6,PCT,CHI3L1,HIF-1α,HA,LN,Ⅳ-C,and PCⅢ in the fibrosis group were higher than those in the non fibrosis group(P＜0.05).The levels of CHI3L1 and HIF-1α were positively correlated with IL-6,PCT,HA,LN,Ⅳ-C,and PC Ⅲ levels(P＜0.05).The AUC of CHI3L1 and HIF-1α in diagnosing liver fibrosis in patients with CHB complicated with liver cirrhosis was 0.810 and 0.801,respectively.The AUC of their combined diagnosis was 0.909,which was bet-ter than their individual diagnosis(Zcombination-CHI3L1=2.893,Zcombination HIF-1α=3.360,P＜0.05).Conclusion The levels of CHI3L1 and HIF-1α are higher in the serum of patients with CHB combined with liver cirrhosis who also had fibrosis,and are positively correlated with liver fibrosis indicators HA,LN,Ⅳ-C,and PC Ⅲ.The combina-tion of the two has high value in diagnosing liver fibrosis in patients with CHB combined with liver cirrhosis,and has certain clinical reference significance.
