Evaluation of the effect of platelet aggregation degree on platelet count by blood smear microscopic examination
10.3969/j.issn.1673-4130.2024.23.004
- VernacularTitle:血涂片镜检法评估血小板聚集程度对血小板计数的影响
- Author:
Hui ZHOU
1
;
Huayang ZHANG
Author Information
1. 首都医科大学附属北京世纪坛医院医学检验科,北京 100038
- Keywords:
blood smear;
microscopic examination;
platelet aggregation degree;
false platelet reduc-tion degree of platelets
- From:
International Journal of Laboratory Medicine
2024;45(23):2834-2837,2843
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the evaluation of platelet aggregation degree through blood smear micros-copy and calculate the correction formula for false decrease in platelet count based on it,in order to achieve ac-curate platelet counting.Methods Venous blood samples with EDTA-K2 anticoagulant causing platelet aggre-gation and sodium citrate anticoagulant not causing platelet aggregation.The manual microscopic examination of blood smears was used to analyze the optimal blood volume and angle for manual slide analysis.Human ma-chine slide comparison and manual slide repeatability comparison were conducted to evaluate the degree of platelet aggregation,its relationship with the degree of false decrease in platelet count was analyzed,and the correction formula for false decrease in platelet count was calculated.Results The results showed that 45° 4μL of artificial smear,and observing 10 oil-immersion fields at the tail junction could more objectively reflect the degree of platelet aggregation.Through fitting of regression equations,platelet aggregation rate,platelet aggregation count,and platelet aggregation pile count showed good linear regression relationships with platelet pseudo-decrease(R2=0.905).Three parameters of platelet aggregation could be obtained through blood smear microscopic examination.The difference in platelet pseudo-decrease could be calculated using the regression e-quation,the sum of the difference and the aggregated EDTA-K2 anticoagulant platelet count result resulted in the corrected platelet count.Conclusion By using blood smear microscopy to calculate platelet aggregation rate,platelet aggregation quantity,and platelet aggregation pile number,three parameters could be effectively corrected for false decrease in platelet count caused by platelet aggregation,achieving accurate platelet count-ing.The operation is simple,economical,and has good clinical and grassroots application value.
