Toxicokinetics of Chlorfenapyr and Its Metabolites in Rats
10.12116/j.issn.1004-5619.2024.340302
- VernacularTitle:虫螨腈及其代谢物在大鼠体内的毒物代谢动力学
- Author:
Wen-Yan LI
1
;
Jin-Feng ZHAO
;
Wei-Chen LIU
;
Shi-Jing LÜ
;
Jia-Xin ZHANG
;
Xu-Dong ZHANG
;
Zhi-Wen WEI
;
Ke-Ming YUN
;
Chao ZHANG
Author Information
1. 山西医科大学法医学院,山西 晋中 030600;山西省法医学重点实验室,山西 晋中 030600;法庭毒物分析公安部重点实验室,山西 晋中 030600
- Keywords:
forensic medicine;
toxicological analysis;
toxicokinetics;
chlorfenapyr;
tralopyril;
gas chroma-tography-mass spectrometry(GC-MS);
liquid chromatography-mass spectroscopy(LC-MS);
rats
- From:
Journal of Forensic Medicine
2025;41(4):380-386
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a chromatography-tandem mass spectrometry method for detecting chlorfenapyr and its metabolite tralopyril in blood,and to investigate the toxicokinetics in rats.Methods Chlorfenapyr(8 mg/kg)was administered orally to rats,and blood samples were collected from rats'canthus vein at 5 min,15 min,30 min,1 h,3 h,6 h,12 h,24 h and 48 h after administration.The blood samples were extracted using 100 μL of 5%formic acid solution and 400 μL of acetonitrile.Chlorfena-pyr was qualitatively and quantitatively detected by triple quadrupole gas chromatography-tandem mass spectrometry(GC-MS/MS)and tralopyril was detected by triple quadrupole liquid chromatography-tandem mass spectrometry(LC-MS/MS).The DAS 3.0 software was used to fit the toxicokinetic equa-tions and calculate the toxicokinetic parameters.Results Chlorfenapyr was detectable from 5 min to 24 h with a peak time of 1 h.Tralopyril was detectable from 15 min to 48 h with a peak time of 3 h.The toxicokinetic process of chlorfenapyr in rat blood conformed to a first-order absorption one-compartment open model,with the toxicokinetic equation described as C=e-0.265t-e-0.175t.Tralopyril con-formed to the first-order absorption three-compartment model,and the toxicokinetic equation was C=47 361.069e-2.209t-35 404.962e-1.486t+11 956.363e-0.512t.In the equations,C stands for the concentration of the target substance in the blood,e is the natural constant(≈2.718 28),and t stands for time.Conclu-sion This study optimized the detection method for chlorfenapyr and its metabolite tralopyril in blood.The toxicokinetic equations and parameters of chlorfenapyr and tralopyril can provide a reference for the estimation of oral intake time of chlorfenapyr.
