Fluorescent Probe Development for Rapid Detection of Tiletamine Based on Cop-per Nanozyme and Molecular Imprinting Technology
10.12116/j.issn.1004-5619.2025.350410
- VernacularTitle:基于铜纳米酶和分子印迹技术的替来他明快速检测荧光探针开发
- Author:
Jia-Hao LI
1
;
Jiang LING
;
Zi-Hao CAI
;
Zi-Yuan ZHENG
;
Yan-Jun DING
Author Information
1. 中南大学湘雅基础医学院法医学系,湖南 长沙 410013
- Keywords:
forensic medicine;
toxicological analysis;
tiletamine;
copper nanozyme;
molecular imprinted polymer;
fluorescent probe;
rapid detection
- From:
Journal of Forensic Medicine
2025;41(4):355-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a rapid detection method for tiletamine that is easy to operate and low-cost under the premise of ensuring sensitivity and accuracy,to assist in carrying out rapid screening and drug control work on-site.Methods This study integrates dual-ligand copper nanozymes with mo-lecular imprinting technology.Initially,copper nanozymes were synthesized using readily available raw materials at 120℃.Subsequently,specific cavities were imprinted on their surface at room temperature using a sol-gel method to construct a novel fluorescent sensing probe.This probe was characterized and methodologically validated,and then applied to the detection of actual samples.Results The developed probe exhibited stable fluorescence properties,strong anti-interference capability,and excellent specificity and sensitivity,with a detection limit of 5 ng/mL and a quantitative concentration range from 15 to 500 ng/mL.It enabled the rapid detection of tiletamine in real samples such as blood and e-cigarette oil.Conclusion This fluorescent probe can be used for rapid detection and on-site preliminary screening of tiletamine in various types of samples.It significantly improves the detection efficiency and reduces analysis costs,showing high research value and broad application prospects.
