Rapid Analysis of Cyanide Based on a Ratiometric Fluorescent Probe Using Gold Nanoclusters-Fluorescein
10.12116/j.issn.1004-5619.2025.350403
- VernacularTitle:基于金纳米团簇-荧光素比率荧光探针的氰化物快速分析
- Author:
Tai-Shen HE
1
;
Zhong-Jiang LÜ
;
Yi-Ming SUN
;
Yu-Yang LI
;
Yi YE
;
Yao LIN
;
Lin-Chuan LIAO
Author Information
1. 四川大学华西基础医学与法医学院,四川 成都 610041
- Keywords:
forensic medicine;
toxicological analysis;
gold nanoclusters(AuNCs);
fluorescein;
cyanide;
ratiometric fluorescent probe;
rapid analysis;
portable device
- From:
Journal of Forensic Medicine
2025;41(4):340-347
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid analysis method for cyanide based on a ratiometric fluores-cent probe,providing a quantitative strategy for on-site visual and rapid detection of cyanide.Methods A dual-emission ratiometric fluorescent probe(AuNCs-FL)was constructed by using bovine serum al-bumin(BSA)-stabilized gold nanoclusters(AuNCs,fluorescence emission at 660 nm)as the responsive signal unit and fluorescein(FL,emission at 515 nm)as the internal reference.Results The etching effect of cyanide on AuNCs resulted in fluorescence quenching at 660 nm,while the fluorescence inten-sity of FL at 515 nm remained unchanged,enabling a rapid response analysis of cyanide shift from red to green fluorescence.The developed probe enabled rapid analysis of cyanide within 3 min,with a limit of detection(LOD)of 3.4 mg/L and a visual detection range of 10-100 mg/L.Conclusion The AuNCs-FL fluorescent probe is structurally simple,low-cost,and easy to operate,delivering rapid and accurate results.It also avoids the interference from sulfides encountered in commercial cyanide test kits,making it suitable for the on-site rapid detection of suspected powder samples in cyanide poisoning cases.
