Temporal Expression of NETosis Marker CitH3 in Deep Vein Thrombosis in Mice
10.12116/j.issn.1004-5619.2024.441004
- VernacularTitle:NETosis标记物CitH3在小鼠深静脉血栓中的时序性表达
- Author:
Qian WANG
1
;
Song-Min YANG
;
Juan-Juan WU
;
Yu ZHANG
;
Xiang-Meng WANG
;
Gang CHEN
;
Peng-Fei JIANG
Author Information
1. 温州医科大学基础医学院,浙江 温州 325035;温州医科大学司法鉴定中心,浙江 温州 325035
- Keywords:
forensic pathology;
deep vein thrombosis;
citrullinated histone H3(CitH3);
temporal expres-sion;
mice
- From:
Journal of Forensic Medicine
2025;41(3):201-207
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression changes of citrullinated histone H3(CitH3)during the development of deep vein thrombosis(DVT)in mice,and to explore its value in estimating the time to thrombosis.Methods The inferior vena cava(IVC)of mice was ligated to establish a thrombosis model induced by congestion.Mice were sacrificed under excessive anesthesia at 0 h,1 d,3 d,5 d,7 d,10 d,14 d and 21 d after the modeling,respectively.The congested IVC segments(0 h after modeling)and the thrombosed IVC segments(1-21 days after modeling)were extracted.Immunohistochemistry and double immunofluorescence staining were used to observe the number of neutrophils and the ex-pression of CitH3 during thrombosis.Western blotting was used to detect the protein expression level of CitH3.Results During thrombosis,CitH3 was mainly expressed in neutrophils within the thrombus.A small number of neutrophils and a few CitH3-positive cells were observed at 0 h after modeling in the congested IVC.Between 1 d and 21 d after modeling,the number of neutrophils reached a peak at 1 d and gradually decreased.The number of CitH3-positive cells and their ratio to neutrophils began to increase at 1 d,reached a peak at 5 d after modeling,and then decreased.The expression level of CitH3 protein began to increase at 1 d and reached a peak at 5 d after modeling.Conclusion The expres-sion of CitH3 during DVI shows temporal changes,and is expected to become a biological marker for estimating the formation time of thrombosis.
