Analysis of In Vitro Mirtazapine Metabolites in Human Liver Microsomes by LC-HRMS
10.12116/j.issn.1004-5619.2024.340606
- VernacularTitle:采用LC-HRMS分析米氮平在人肝微粒体中的体外代谢产物
- Author:
Ying ZHANG
1
;
Wen-Fang ZHANG
;
Duo-Qi XU
;
Shi-Yang QIN
;
Shi-Yun YANG
;
Jing QIAO
Author Information
1. 北京市公安司法鉴定中心 法庭毒物分析公安部重点实验室,北京 100192
- Keywords:
forensic medicine;
toxicological analysis;
mirtazapine;
human liver microsome;
metabolite;
metabolic pathway;
liquid chromatography-high resolution mass spectrometry(LC-HRMS)
- From:
Journal of Forensic Medicine
2024;40(6):569-574
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and optimize an in vitro incubation system with human liver micro-somes and investigate the in vitro metabolites and possible metabolic pathways of mirtazapine.Methods Three major metabolites of mirtazapine were selected to optimize the incubation conditions of liver mi-crosomes.The metabolites of mirtazapine were analyzed by liquid chromatography-high resolution mass spectrometry(LC-HRMS)to identify the in vitro metabolites and metabolic pathways of mir-tazapine.Results Ten metabolites,including nine phase Ⅰ metabolites and one phase Ⅱ metabolite,were identified in the in vitro liver microsome incubation.Among them,five new metabolites and one new metabolic pathway were discovered.The pathways involved in phase Ⅰ metabolic included methyla-tion,hydroxylation,oxidation,reduction,etc.,while the phase Ⅱ biotransformation was mainly glucuroni-dation.Conclusion The metabolites discovered in this study are consistent with the main metabolites of mirtazapine reported in literature,which are N-desmethylmetazapine,8-hydroxy mirtazapine and mirtazapine-N-oxide.The results can provide basis for the confirmation of mirtazapine cases and pro-vide reference for the study of other substances.
