Rapid Screening of 60 Types of Natural Toxins in Whole Blood by UPLC-Q/Orbitrap HRMS
10.12116/j.issn.1004-5619.2024.340202
- VernacularTitle:UPLC-Q/Orbitrap HRMS对全血中60种天然毒素的快速筛查
- Author:
Zhu-Tao GUO
1
;
Qian-Ya DENG
;
Si-Yang HE
;
Shui-Qing ZHENG
;
Rong WANG
;
Wen-Juan SUN
;
Chen LIANG
;
Chun-Fang NI
Author Information
1. 上海市公安局物证鉴定中心 上海市现场物证重点实验室,上海 200083;复旦大学药学院,上海 201203
- Keywords:
forensic medicine;
toxicological analysis;
ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q/Orbitrap HRMS);
natu-ral toxin;
whole blood;
rapid screening
- From:
Journal of Forensic Medicine
2024;40(6):564-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid screening method for 60 types of natural toxins in whole blood by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high reso-lution mass spectrometry(UPLC-Q/Orbitrap HRMS).Methods The chromatographic and mass spectro-metric information of 60 standard samples of natural toxins were analyzed and recorded,and a screening database was built.Whole blood was pretreated by protein precipitation method combined with ultrasonic-assisted dispersion,and then a Hypersil GOLDTM C18 column was used with 5 mmol/L ammonium for-mate aqueous solution(containing 0.1%formic acid)and acetonitrile as mobile phase for gradient elu-tion.In the positive ion mode,the data were collected in full scan/data-dependent secondary scan(Full MS/dd-MS2)mode.Based on the established screening library,the rapid screening of 60 types of natural toxins in whole blood was realized by TraceFinder software.Results A UPLC-Q/Orbitrap HRMS method was developed for the screening of 60 types of natural toxins in whole blood.Except for the limit of detection(LOD)of oxymatrine(20 ng/mL)and strophanthidin(40 ng/mL),the LOD for the other 58 natural toxins was in the range of 0.05-5 ng/mL.Conclusion This method has a simple and efficient pretreatment process and can achieve rapid screening of 60 types of natural toxins in whole blood.
