High Resolution Mass Spectrometry Fragmentation Rules of Etomidate and Its Structural Analogues
10.12116/j.issn.1004-5619.2024.340602
- VernacularTitle:依托咪酯及其结构类似物的高分辨质谱裂解规律
- Author:
Xian-Yu FAN
1
;
Xue-Yan ZHU
;
Xin WANG
;
Ping XIANG
;
Jun-Bo ZHAO
Author Information
1. 司法鉴定科学研究院 上海市法医学重点实验室 司法部司法鉴定重点实验室 上海市司法鉴定专业技术服务平台,上海 200063
- Keywords:
forensic medicine;
toxicological analysis;
etomidate;
metomidate;
isopropoxate;
propoxate;
high resolution mass spectrometry(HRMS);
gas chromatography(GC);
liquid chromatography(LC);
fragmentation rule
- From:
Journal of Forensic Medicine
2024;40(6):557-563
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the characteristic fragment ions,ion abundance ratios and mass spec-trometry fragmentation rules of etomidate and its structural analogues by using gas chromatography-quadrupole/orbitrap mass spectrometry(GC-Q/Orbitrap MS)and liquid chromatography-linear ion trap quadrupole-orbitrap mass spectrometry(LC-LTQ-Orbitrap MS)techniques,providing important data support for the identification and prediction of etomidate structural analogues.Methods GC-Q/Orbitrap MS and LC-LTQ-Orbitrap MS were used to analyze metomidate,etomidate,isopropoxate and propoxate,to obtain their GC high resolution mass spectra and LC high resolution mass spectra.Results Under the electron impact(EI)ion source mode,etomidate,metomidate and the other two analogues all pro-duced their molecular ions and seven identical fragment ions(m/z 77.038 6,79.054 2,95.024 0,105.069 9,143.073 0,172.099 5 and 199.086 6),among which isopropoxate and propoxate also produced characteris-tic fragment ions m/z 216.089 3.In the collision cell of the electrospray ionization(ESI)source mode,etomidate,metomidate and the other two analogues all produced three identical fragment ions(m/z 95.024 0,105.069 9 and 113.034 6).Meanwhile,each substance produced one characteristic fragment ion(metomidate:m/z 127.050 2;etomidate:m/z 141.065 9;isopropoxate and propoxate:m/z 155.081 5).Con-clusion Common fragment ions exist among etomidate and its structural analogues,and characteristic ion fragments are generated based on the different carbon numbers of their side chains.The structure of side chains can affect the abundance ratio of each fragment ion,providing a basis for the structural identification and prediction of such compounds.
