Test and Evaluation of the Made-in-China Quick TargSeq Fully Integrated Au-tomated On-site Rapid DNA Detection Instrument
10.12116/j.issn.1004-5619.2023.530601
- VernacularTitle:国产Quick TargSeq全集成自动化现场快速DNA检测仪的测试评估
- Author:
Jun-Ping HAN
1
;
Ying-Xi WANG
;
Chao LIU
;
Feng LIU
;
Jin-Rong GUO
;
Wei ZHU
;
Guang-Feng TANG
;
Cai-Xia LI
;
Xin WANG
;
Zhang-Ping JIAO
Author Information
1. 北京市公安局朝阳分局刑事侦查支队,北京 100025
- Keywords:
forensic genetics;
forensic DNA typing;
rapid DNA analysis;
short tandem repeat;
ancestry inference
- From:
Journal of Forensic Medicine
2024;40(5):461-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To verify the stability and accuracy of the made-in-China Quick TargSeq Fully Integrated Automated On-site Rapid DNA Detection Instrument(hereinafter referred to as Quick Targ-Seq DNA detection instrument)and evaluate its forensic application prospects.Methods A total of 74 blind samples provided by the expert group of Criminal Investigation Bureau of the Ministry of Public Security(4 individuals,20 samples)and five participating institutions(54 samples)were used to test on 5 Quick TargSeq DNA detection instruments and the accompanying microfluidic chip cartridges.The results were compared with the known genotypes to calculate the samples'detection rate and genotyping accuracy.Results The results of reproducibility obtained from 20 samples provided by the expert group on the 5 Quick TargSeq DNA detection instruments were all consistent with the known genotypes.The detection rate of the 74 samples was 100%,and the genotyping accuracy was 97.33%.Conclusion The Quick TargSeq DNA detection instrument has good stability and high genotyping accu-racy.
