Identification of Antemortem and Postmortem Injuries in Nude Mice Based on Microbial Communities
10.12116/j.issn.1004-5619.2023.430806
- VernacularTitle:基于细菌群落鉴别裸鼠生前损伤和死后损伤
- Author:
Xin ZHENG
1
;
Yue QIU
;
Zhi-Gang LI
;
Qing-Qing XIANG
;
Guan-San WANG
;
He SHI
;
Qu-Yi XU
;
Peng SUI
;
Yan-Bing MA
;
Chao LIU
;
Li-Fang CHEN
;
Jian ZHAO
Author Information
1. 昆明医科大学法医学院,云南 昆明 650500
- Keywords:
forensic pathology;
microbiology;
colony;
antemortem injury;
postmortem injury;
16S rRNA;
BALB/c nude mice
- From:
Journal of Forensic Medicine
2024;40(5):430-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish antemortem and postmortem injury models in nude mice,observe the morphological changes of the wounds and the changes of the microbial communities in the wounds at different time points after the injury,and analyze the differences between antemortem and postmor-tem wounds.Methods Abdominal injury models were established in 48 BALB/c nude mice,which were classified into antemortem injury,4 h and 72 h postmortem injury groups,and the gross manifesta-tions and histopathological changes were observed on days 1,3,5,8,11 and 15 after injury.The mi-crobial communities in the wounds were analyzed by 16S rRNA sequencing technology.QIIME 2 soft-ware was used to calculate Shannon and Observed species indices.The Kruskal-Wallis test was used to determine statistical differences in α-diversity between groups.Jaccard similarity coefficients were calculated by using R v4.3.0 software and applied to the principal co-ordinates analysis to demonstrate inter-sample differences.Permutational multivariate analysis of variance(PERMANOVA)was used to analyze the differences between groups in the composition of bacterial colonies,and R2 values were calculated.Results On days 8,11 and 15 after injury,the antemortem and postmortem injuries could not be differentiated by morphological examination;the Shannon index and Observed species index were statistically different between the antemortem injury group and the 72 h postmortem injury group;the Jaccard similarity coefficient of the microbial community was statistically different between the an-temortem injury group and the 72 h postmortem injury group.The PERMANOVA R2 value gradually increased with the extension of time(0.22-0.61).Conclusion Through the analysis of the wound mi-crobial community,the microbial composition of wounds at different time points can be identified and compared,which provides a new perspective and method for the differentiation of antemortem injuries from postmortem injuries,with good application prospects.
