Porous Mn,Zr-CeO2 Nanozymes-based Cascade Catalytic Amplification for Electrochemical Analysis of miRNA-21 at Physiological pH
10.19756/j.issn.0253-3820.241411
- VernacularTitle:基于多孔Mn,Zr-CeO2纳米酶的级联催化放大用于生理pH条件下miRNA-21的电化学分析
- Author:
Xiao-Xing XU
1
;
Lan GU
;
Rong-Qi ZHANG
;
Bei-Bei KOU
;
Xiao-Yong JIN
Author Information
1. 宁夏大学化学化工学院,银川 750021
- Keywords:
Mn,Zr-CeO2 nanozyme;
Efficiency of enzyme cascade catalysis;
Signal amplification;
Electrochemical biosensor
- From:
Chinese Journal of Analytical Chemistry
2025;53(11):1940-1952,后插1-后插2
- CountryChina
- Language:Chinese
-
Abstract:
Enzymatic cascade catalysis has emerged as an effective means to enhance the sensitivity of biosensors due to its remarkable amplification effect on electrochemical signals.However,the most used natural enzymes have high specificity and high catalytic activity,but are susceptible to environmental factors,easy denaturation and inactivation,and high cost,which limit their practical applications.Additionally,the majority of nanozymes with excellent catalytic activity cannot be directly used as redox probes.The redox signal can only be required under high potentials in strong acid/alkali solutions,or functionalized with electroactive substances.To tackle this problem,herein,AuNPs(glucose oxidase-like activity)and Mn,Zr dual-doped CeO2 nanozymes(Mn,Zr-CeO2,peroxidase-like activity)were used as model enzymes to construct a high-performance nanozymes cascade catalytic system.Owing to high Ce4+/Ce3+ratio and a considerable number of oxygen vacancies,Mn,Zr-CeO2 nanozymes exhibited excellent peroxidase-like activity and could generate amplified electrochemical signals in neutral medium at low potentials.Furthermore,the porous structure of Mn,Zr-CeO2 nanozymes could accelerate the mass transfer of intermediate H2O2,thereby enhancing the efficiency of enzymatic cascade catalysis.As a result,a label-free electrochemical biosensor was constructed for sensitive detection of the cancer marker miRNA-21 at physiological pH,with a detection limit as low as 32.5 fmol/L.This strategy offered a novel approach for the development of a new generation of high-performance nanozymes cascade platforms,which could be widely applied in the fields such as biotechnology,bioanalysis,and disease diagnosis.
