Dual-modal Ultraviolet-Fluorescence Sensor Based on Acetaldehyde Dehydrogenase/Bovine Serum Albumin System for Acetaldehyde Detection in Food
10.19756/j.issn.0253-3820.251245
- VernacularTitle:基于乙醛脱氢酶/牛血清白蛋白体系的紫外荧光双模态传感器用于食品中乙醛的检测
- Author:
Feng LI
1
;
Ya-Hao LIU
;
Kun GE
;
Lun-Zhao YI
Author Information
1. 昆明理工大学食品科学与工程学院,昆明 650500
- Keywords:
Acetaldehyde;
Aldehyde dehydrogenase;
Bovine serum albumin;
Ultraviolet detection;
Fluorescent detection
- From:
Chinese Journal of Analytical Chemistry
2025;53(11):1931-1939,中插53-中插57
- CountryChina
- Language:Chinese
-
Abstract:
In this study,an ultraviolet(UV)/fluorescence dual-modal sensor was constructed by combining the catalytic properties of aldehyde dehydrogenase(ALDH)with the fluorescence inner filter effect of bovine serum albumin(BSA),realizing highly sensitive and highly selective detection of acetaldehyde in food.Acetaldehyde could react with oxidized coenzyme I(NAD+)to generate acetic acid and reduced coenzyme I(NADH)under catalytic condition by ALDH,and a quantitative relationship between acetaldehyde concentration and UV signal for UV detection based on the characteristic UV absorption peak of NADH at 340 nm was established.Meanwhile,an acetaldehyde detection channel based on fluorescence signal changes was built on the basis of the property that the fluorescence emission of BSA(as a fluorescent indicator)at 340 nm could be effectively quenched by the generated NADH,thus forming a″one-reaction dual-signal″detection mode.The experimental results showed that the acetaldehyde concentration in the range of 0.01?5.0 mg/L had a good linear relationship with both UV and fluorescence signals,and the limit of detection(LOD)was 0.003 mg/L for the UV mode and 0.005 mg/L for the fluorescence mode,and no significant fluctuations were observed when 50-fold concentrations of acetaldehyde analogs and common interfering substances were added.When the dual-modal sensor was applied to detection of acetaldehyde in food samples such as yogurt and wine,the relative error between its quantitative results and those of high-performance liquid chromatography(HPLC)was less than±4.0%.The dual-modal cross-validation strategy could improve the detection reliability through signal mutual verification,providing an innovative solution for the rapid detection of acetaldehyde contamination in food.
