A Colorimetric and Electrochemical Dual-Mode Modified Enzyme-linked Immunosorbent Assay Method Based on Calcium Carbonate Nanocubes
10.19756/j.issn.0253-3820.251067
- VernacularTitle:基于碳酸钙纳米立方体的光电双模式改良型酶联免疫吸附测定方法的研究
- Author:
Huan-Ming CUI
1
;
Min MAO
;
Xun MAO
Author Information
1. 中南林业科技大学化学与化工学院,长沙 410004
- Keywords:
Calcium carbonate nanocubes;
Colorimetry;
Electrochemical immunoassay;
Human ferritin;
Lung cancer
- From:
Chinese Journal of Analytical Chemistry
2025;53(11):1839-1847
- CountryChina
- Language:Chinese
-
Abstract:
In the study,calcium carbonate nanocubes(CCNC)were controllably prepared,and a simple and universal modified enzyme-linked immunosorbent assay(ELISA)method was developed for colorimetric and electrochemical dual-mode detection of lung cancer potential biomarker ferritin.The classic sandwich immunoassay mode was adopted.Monoclonal antibodies were modified on microwell of microplate and CCNC firstly.In the presence of ferritin,the CCNC were well captured on the surface of the microplate through the sandwich immunoreaction mode.Calcium ions released by acid hydrolysis formed a colored complex with the following added chromogenic agent,and the color of the sample solution changed from weak yellow to red.Spectrophotometry could be used for the quantitative detection of ferritin.Under the optimal conditions,the absorbance at 512 nm showed a good linear relationship with the logarithm of concentration of ferritin in the range of 1-250 ng/mL,with detection limit of 0.6 ng/mL,which was comparable that of the traditional ELISA method.In addition,gold nanoparticles(AuNPs)modified electrode-based electrochemical technique was also been introduced into the modified ELISA method.Under the optimized conditions,the linear range for ferritin test was 0.05-100 ng/mL,with an extreme low detection limit of 32 pg/mL(S/N>3).The CCNC probe used here exhibited obvious advantages over the enzyme probes in traditional ELISA technique in terms of stability,simplicity and cost,providing a new type of nanoprobe for ELISA technology.
