Nanochannels Electrochemical Biosensing Method Based on Dual Electrochemical Probes and Its Application in Detection of Carcinoembryonic Antigen
10.19756/j.issn.0253-3820.251195
- VernacularTitle:基于双电化学探针的纳米通道生物传感方法用于癌胚抗原检测
- Author:
Jiao JIAO
1
;
Hong-Fang ZHANG
Author Information
1. 西北大学化学与材料科学学院,西安 710127;运城学院化学系,运城 044300
- Keywords:
Potassium hexacyanoferrate;
Methylene blue;
Carcinoembryonic antigen;
Nanochannel;
Dual probes;
Self-calibrating
- From:
Chinese Journal of Analytical Chemistry
2025;53(10):1642-1653
- CountryChina
- Language:Chinese
-
Abstract:
A self-calibrating nanochannels electrochemical biosensing method for carcinoembryonic antigen(CEA)was established using two electrochemical probes,potassium hexacyanoferrate(K3[Fe(CN)6])and methylene blue(MB).Firstly,the electrochemical behaviors of K3[Fe(CN)6]and MB solutions were investigated,and it was found that these two probes could easily interact to form complexes.Based on this,K3[Fe(CN)6]and MB were used as electrochemical signal probes,respectively,CEA antibody-functionalized anodic aluminum oxide(AAO)nanochannels as recognition units,and DNA self-assembled hybridization chain reaction(HCR)as a signal amplification strategy to improve the sensitivity and accuracy of the detection.A self-calibrating nanochannels electrochemical biosensing method based on the two electrochemical probes was thus established.The optimal experimental conditions were 1.5 h of target incubation time,1 h of CEA aptamer incubation time,0.5 μmol/L of hybridization chain concentration and 2 h of hybridization chain reaction time.The linear range of this method for CEA detection was 0.01 ng/mL to 100 ng/mL,and the detection limits(S/N=3)of K3[Fe(CN)6]and MB as probes were 8.2 and 2.0 pg/mL,respectively.The recoveries for CEA determination in rat serum were closer to 100%after calibration.In addition,the established electrochemical biosensing method had good specificity,repeatability and selectivity.This study provided a new idea for improving the accuracy of nanochannel electrochemical biosensing analysis.
