Systematic Optimization of Swelling-Enzymatic Extraction and Aqueous Two-Phase Purification for Phycocyanin
10.19756/j.issn.0253-3820.251130
- VernacularTitle:溶胀-酶解联用提取藻蓝蛋白及双水相纯化工艺的建立和系统优化
- Author:
Jia-Rong LIN
1
;
Shun-Yi WANG
;
Fang LUO
;
Cui-Ying LIN
;
Zhen-Yu LIN
Author Information
1. 福州大学生物科学与工程学院,福州 350116
- Keywords:
Phycocyanin;
Extraction process;
Aqueous two-phase extraction;
Separation and purification
- From:
Chinese Journal of Analytical Chemistry
2025;53(9):1516-1525
- CountryChina
- Language:Chinese
-
Abstract:
An integrated extraction-purification process was established in this work for efficient phycocyanin production from dried Spirulina platensispowder.Initially,phycocyanin was extracted from algal biomass using a combined swelling-enzymatic lysis strategy.Single-factor experiments were carried out to systematically evaluate the effects of critical parameters,including system pH,enzymatic hydrolysis duration,and temperature,on phycocyanin extraction yield.Subsequently,a three-factor,three-level Box-Behnken response surface methodology design was employed to optimize the extraction process,with phycocyanin concentration and purity in the extract serving as response variables.A predictive regression model identified optimal conditions as follows:pH 6.4,hydrolysis time 3.2 h,and temperature 35.4℃.Experimental validation under these conditions yielded a phycocyanin recovery of 26.6%.Following extraction,purification was achieved via a polyethylene glycol-phosphate aqueous two-phase extraction system,which elevated the final purity to 4.21.Results demonstrated that the swelling-enzymatic lysis approach effectively disrupted algal cellular structures,significantly enhancing phycocyanin release efficiency.Concurrently,the aqueous two-phase system enabled selective partitioning and enrichment of the target protein under mild conditions.The integrated process exhibited high extraction efficiency,gentle purification,and robust operability,rendering it suitable for the scalable production of natural phycocyanin.This work provided both methodological foundations and technical support for advancing phycocyanin applications in natural pigments,biomedicine,and analytical detection.
