Impact of Multi-Component Fatty Acid Calibration Methods on Quantification of Infant Formula
10.19756/j.issn.0253-3820.251140
- VernacularTitle:婴幼儿配方乳粉中多组分脂肪酸校准方法对测量的影响
- Author:
Xiao-Ting CHEN
1
;
Meng-Qian XU
;
Qing-He ZHANG
;
Xiu-Qin LI
Author Information
1. 齐齐哈尔医学院公共卫生学院,齐齐哈尔 161006
- Keywords:
Fatty acid;
Infant formula;
Quantitative calibration method;
Gas chromatography flame ionization detector
- From:
Chinese Journal of Analytical Chemistry
2025;53(9):1505-1515,中插4-中插5
- CountryChina
- Language:Chinese
-
Abstract:
Infant formula milk powder(infant formula)contains a variety of fatty acids that require hydrolysis and derivation,and the calibration methods are complex and variable,affecting the accuracy of quantification.Using the gas chromatography with flame ionization detector(GC-FID)under internal standard and external standard calibration methods,the effects of using fatty acid methyl esters(FAMEs)and triacylglycerol(TAGs)as calibration solutions on determination of 19 kinds of fatty acids content in infant formula were compared in this work.Additionally,the differences in determination between the acetyl chloride methanol method and the hydrolysis extraction method using FAMEs as the calibration solution under internal standard method were also compared.The quantitative results of TAGs external standard quantitative method were significantly higher than those of FAMEs external standard quantitative method and FAMEs calibrator derived by methylation,and the deviation of quantitative results were 7%-20%and 2%-10%,respectively.The quantitative results of FAMEs calibrator added FAME internal standard were significantly higher than those of FAMEs calibrator added FAME internal standard derived from TAG methylation and FAMEs calibrator added TAG internal standard with methyl esterification,and the deviation of quantitative results were about 15%and 2%-5%,respectively.The results indicated that both the two methods of internal standard calibration using FAMEs as the calibration solution and external standard calibration using TAGs as the calibration solution could effectively eliminate the bias in the determination,with simple operation and accurate comparation of the results.However,the results were significantly lower under the external standard calibration using FAMEs as calibration solution.
