Application of Fluorescence Sensor Based on H+-Induced i-Motif in Microalgae CO2 Fixation Technology
10.19756/j.issn.0253-3820.251031
- VernacularTitle:基于H+诱导i-Motif的荧光传感器在微藻固定CO2技术中的应用
- Author:
Zhe-Han YANG
1
;
Yu-Han WANG
;
Zhen-Jia YANG
;
Jie YIN
Author Information
1. 重庆工商大学环境与资源学院,重庆 400067
- Keywords:
Microalgae carbon capture and conversion;
Carbonic anhydrase;
Fluorescent sensor;
DNA probe;
i-Motif structure
- From:
Chinese Journal of Analytical Chemistry
2025;53(9):1496-1504
- CountryChina
- Language:Chinese
-
Abstract:
Microalgae can achieve sustainable CO2 fixation and biomass conversion,making them a green path for carbon capture and conversion.Monitoring extracellular carbonic anhydrase(CA)activity in microalgae is crucial for regulating carbon conversion efficiency.In this study,double-stranded DNA was designed as a capture probe to bind H+,which was produced by CA-catalyzed CO2 hydration.This interaction induced conformational changes in the DNA probe,enhancing fluorescence signals and thereby establishing a recognition and signal transduction mechanism for CA catalytic product.This approach enabled a novel method for measuring CA activity.The response of probe to H+was investigated using ultraviolet visible(UV-Vis)spectroscopy,circular dichroism sepctroscopy,and fluorescence spectroscopy.The experimental results indicated that the fluorescence intensity of the probe increased progressively with rising H+concentration.Using this probe,CA activity detection could be completed within 29 min,with a detection range of 0.35-17500 U/mL and a detection limit of 0.15 U/mL.Compared to traditional methods,this approach offered a lower detection limit and was suitable for analyzing and detecting extracellular CA activity in actual algal samples.
