One-pot Synthesis of Sulfhydryl-protected Fluorescent Silver Nanoclusters and Its Application in Detection of Tetracycline
10.19756/j.issn.0253-3820.251136
- VernacularTitle:一锅法合成巯基保护的荧光银纳米簇用于检测四环素
- Author:
Xi-Liang YANG
1
;
Ya-Ya KUANG
;
Zi-Tao LI
;
Qiu-E CAO
;
Chuan-Hua ZHOU
Author Information
1. 云南大学化学科学与工程学院,化学化工国家级实验教学示范中心,昆明 650500
- Keywords:
Silver nanoclusters;
Tetracycline;
Fluorescence detection;
Nanomaterials
- From:
Chinese Journal of Analytical Chemistry
2025;53(9):1486-1495
- CountryChina
- Language:Chinese
-
Abstract:
Water-soluble silver nanoclusters(DHLA-AgNCs)with red fluorescence emission were synthesized using silver nitrate(AgNO3)as silver source,dihydrolipoic acid(DHLA)as ligand and sodium borohydride(NaBH4)as reducing agent by one-pot method.Based on the selective quenching of DHLA-AgNCs by tetracycline(TC),a rapid and selective fluorescence analysis method for detection of TC was constructed by monitoring the fluorescence intensity change at 650 nm.Under the optimal detection conditions,the fluorescence quenching efficiency of DHLA-AgNCs showed good linear relationship with concentration of TC within the range of 10.0-120.0 μmol/L,and the limit of detection(LOD)was 0.39 μmol/L.This method was successfully applied to detection of TC in milk samples,with spiked recoveries ranging from 99.5%to 102.5%,and relative standard deviations(RSDs)less than 5%.This method had the advantages of simplicity,rapidity,strong specificity and low cost,and thus provided a simple and feasible strategy for selective detection of TC.
