Colorimetric Sensor for Determination of Golgi Protein 73 Based on Hemin-Reduced Graphene Oxide-Manganese Dioxide Nanozyme
10.19756/j.issn.0253-3820.241439
- VernacularTitle:基于氯化血红素-还原氧化石墨烯-二氧化锰纳米酶的比色传感器检测高尔基体蛋白73
- Author:
Xiao-Hong TAN
1
;
Jia-Hao ZHOU
;
Pei-Hong XU
;
Hao LIN
;
Gui-Yin LI
Author Information
1. 广东石油化工学院化学学院,茂名 525000
- Keywords:
Nanozyme;
Colorimetric sensor;
Hepatocellular carcinoma;
Golgi protein 73;
Peroxidase-like activity
- From:
Chinese Journal of Analytical Chemistry
2025;53(9):1476-1485
- CountryChina
- Language:Chinese
-
Abstract:
Hepatocellular carcinoma(HCC)is one of common cancer that seriously endangers human health.Designing methods for early,rapid,and accurate diagnosis of HCC has become the key point.Golgi protein 73(GP73),a novel potential biomarker for HCC,is crucial for diagnosis and treatment of HCC.In this study,a colorimetric sensor with rapidity,smplicity and high specificity was established for detection of GP73 based on peroxidase-like activity of hemin-reduced graphene oxide-manganese dioxide(H-rGO-MnO2).The H-rGO-MnO2-GP73Apt1 signal probe was synthesized by carboxyl of H-rGO-MnO2 nanozyme and amination of GP73 aptamer(GP73Apt1)though amide reaction.In the presence of GP73,the sulfhydryl-modifed GP73 aptamer(GP73Apt2),as the capture probe,and the signal probe both specifically recognized GP73,forming a sandwich structure(GP73Apt2-GP73-H-rGO-MnO2-GP73Apt1).This structure could catalyze the oxidation of H2O2 to produce hydroxyl radical(·OH),thereby oxidizing the colorless phthalenediamine(OPD)into the yellow 2,3-diaminophenazine(DPA).The quantitative detection of GP73 was achieved by measuring the characteristic absorbance of DPA at 450 nm.In the GP73 concentration range of 10-150 ng/mL,there was a good linear relationship between the DPA absorbance at 450 nm(A450 nm)and the GP73 concentration under optimal conditions.The linear equation was A450 nm=0.00321CGP73+0.8988,with the correlation coefficient(R2)of 0.9960 and the detection limit(LOD)of 5.38 ng/mL.The colorimetric sensor was applied to detection of GP73 in human serum samples,with recoveries of 88.4%?98.8%.This sensor showed high specificity,sensitivity,and stability,and had potential for clinical detection of GP73,providing a new approach for the early diagnosis of HCC.
