A Sensitive Lateral Flow Immunoassay for Detection of Interleukin-6 Using Carbon Dots-Mesoporous Silica Nanocomposite Fluorescent Probes
10.19756/j.issn.0253-3820.251156
- VernacularTitle:基于碳点-介孔二氧化硅纳米荧光探针的高灵敏侧流免疫分析平台检测白细胞介素-6
- Author:
Yue-Qian YANG
1
;
Peng-Yue WANG
;
Jia-Qi REN
;
Xiao PAN
;
Feng-Hua TAN
;
Yu-Jie MA
;
Cong-Ying WEN
;
Jing-Bin ZENG
Author Information
1. 中国石油大学(华东)化学化工学院,化学品安全全国重点实验室,青岛 266580
- Keywords:
Carbon dots;
Mesoporous silica;
Lateral flow immunoassay;
Interleukin-6
- From:
Chinese Journal of Analytical Chemistry
2025;53(9):1467-1475
- CountryChina
- Language:Chinese
-
Abstract:
In this study,a sensitive lateral flow immunoassay(LFIA)platform based on carbon dots-mesoporous silica nanocomposite(CD-MSNs)fluorescent probes was constructed for high-performance detection of inflammatory marker interleukin-6(IL-6).Green fluorescent carbon dots(CDs)were prepared by hydrothermal method with 3,9-perylenic acid and 3-aminopropyltriethoxysilane(APTES)as raw materials,and highly fluorescent CD-MSNs composites were then constructed by encapsulating the prepared CDs in mesoporous silica nanoparticles(MSNs).Fluorescent probes were prepared by covalent coupling of CD-MSNs with IL-6 antibody.Fluorescent immunochromatographic test strips were constructed by spraying IL-6 capture antibody and goat anti-mouse IgG on nitrocellulose membrane as detection line(T-line)and quality control line(C-line),respectively.The fluorescence immunoassay analyzer was used to quantitatively detect the fluorescence intensity of T-line,and the experimental results showed that the LFIA platform based on this probe had a good linear relationship in IL-6 concentration range of 102-106 pg/mL,and the detection limit was 64 pg/mL,which was two orders of magnitude more sensitive than that of the traditional colloidal gold test strips.This method effectively solved the issue of insufficient sensitivity of traditional LFIA technique,and provided a rapid and highly sensitive detection method for early diagnosis of inflammatory diseases.
