AuNPs-FeCDs Dual Nanozyme Cascade System Integrated with A Smartphone Platform for Sensitive Detection of Glucose
10.19756/j.issn.0253-3820.251155
- VernacularTitle:基于AuNPs-FeCDs双纳米酶级联体系结合智能手机的葡萄糖高灵敏检测方法
- Author:
Qing-Jing YE
1
;
Xue-Ying ZHOU
;
Yan-Ying ZHENG
;
Yun ZHANG
;
Wen-Ying JIN
;
Ya-Li YUAN
Author Information
1. 桂林理工大学化学与生物工程学院,桂林 541004
- Keywords:
Gold nanoparticles;
Carbon dots;
Nanozymes;
Cascade system;
Glucose detection
- From:
Chinese Journal of Analytical Chemistry
2025;53(9):1457-1466
- CountryChina
- Language:Chinese
-
Abstract:
A centrifugation-free,single-reaction colorimetric method for detection of glucose,utilizing a dual nanozyme cascade system based on gold nanoparticles(AuNPs)and iron-doped carbon dots(FeCDs),was developed in this work.The AuNPs exhibited glucose oxidase-like activity to catalyze glucose oxidation for generation of H2O2,while the FeCDs demonstrated peroxidase-like activity to subsequently catalyze the H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine(TMB).To prevent interference from the blue signal generated by self-aggregation of AuNPs in subsequent quantitative detection,the reaction system was terminated with HCl,converting oxTMB into a stable yellow product.Based on changes in the absorbance at 450 nm of this yellow solution,a quantitative relationship was established between glucose concentration and absorbance at 450 nm(A450).Experimental results demonstrated that this sensor achieved a linear detection range of 44 μmol/L to 11.11 mmol/L(R2=0.993)with a detection limit of 30.68 μmol/L and spiked recoveries of 97.9%-104.7%.By integrating smartphone-based color recognition capabilities,a rapid visual detection platform was established for quantification of glucose through RGB analysis.The validation experimental results using commercial glucose injection samples further confirmed the practical application potential of this methodology.
