Advances in Microfluidic Chip Based on Loop-mediated Isothermal Amplification Technology for Rapid Detection of Foodborne Pathogens
10.19756/j.issn.0253-3820.251181
- VernacularTitle:基于环介导等温扩增技术的微流控芯片在食源性致病菌快速检测中的研究进展
- Author:
Yan-Zhu PAN
1
;
Jia-Hua TANG
;
Yue-Xiang CHAI
;
Lu-Xi SHU
;
Jia-Xin XIE
;
Yan ZHANG
Author Information
1. 昆明理工大学理学院,昆明 650500
- Keywords:
Loop-mediated isothermal amplification;
Microfluidic chip;
Foodborne pathogens;
Rapid detection;
Review
- From:
Chinese Journal of Analytical Chemistry
2025;53(9):1447-1456
- CountryChina
- Language:Chinese
-
Abstract:
Loop-mediated isothermal amplification(LAMP)is a newin vitronucleic acid amplification technique,which has been widely used in rapid detection of pathogenic bacteria,with advantages of high efficiency,simple operation and low cost.Microfluidic chip technique is a kind of miniaturized and integrated analytical technology,which integrates automation and high throughput,and can effectively avoid sample cross-contamination and has the advantages of high sample utilization rate and high cost-effectivenes.LAMP combined with microfluidic chip can detect multiple samples of the same pathogen at the same time or single samples of different pathogens at the same time,providing a new method for the rapid,field detection of pathogens.In this paper,the research progresses of LAMP microfluidic chip and its application in rapid detection of pathogenic bacteria in recent years were reviewed,and the future prospect was discussed.
