Progress in Methods for Electrochemical Detection of Thrombin
10.19756/j.issn.0253-3820.251172
- VernacularTitle:凝血酶电化学检测方法的研究进展
- Author:
Di WU
1
;
Xi-Yao ZHANG
;
Jing-Jing XU
;
Yi-Ting CHEN
;
Wen-Qi TANG
;
Wen-Hui XU
;
Song-Min CHEN
;
Qiong HU
;
Li NIU
Author Information
1. 广州大学化学化工学院,广州大学分析科学技术研究中心,广州市传感材料与器件重点实验室,广东省光电传感材料与器件工程技术研究中心,广州 510006
- Keywords:
Thrombin;
Electrochemical biosensor;
Nucleic acid aptamer;
Substrate peptide;
Review
- From:
Chinese Journal of Analytical Chemistry
2025;53(9):1403-1410
- CountryChina
- Language:Chinese
-
Abstract:
As a serine protease,thrombin can convert soluble fibrinogen into insoluble fibrin and plays a pivotal role in the coagulation cascade.Therefore,the accurate quantitative assay of thrombin levels is of great value in the evaluation of coagulation function,clinical screening and prognostic monitoring of coagulation-related diseases,and screening of drugs for targeted therapy.Existing methods for thrombin detection can be divided into two categories,e.g.,the assay of concentration levels using nucleic acid aptamers as the affinity elements and the assay of activity levels based on the hydrolytic cleavage of substrate peptides.In recent years,electrochemical biosensors have attracted much attention in thrombin detection due to high sensitivity,high selectivity,simple instrument,fast response,and good portability.In this review,the latest research progress in methods for electrochemical detection of thrombin was summarized,focusing on the detection principles and the applied signal amplification strategies of related electrochemical biosensors.In addition,the challenges with respect to the practical use of electrochemical thrombin biosensors and the prospects were discussed.
