Research on Targeted Screening of Diflorasone Components in Health Products Using Feature Ion Guided Strategy Combined with High-Resolution Mass Spectrometry
10.19756/j.issn.0253-3820.241446
- VernacularTitle:特征离子导向策略结合高分辨质谱技术在保健品二氟拉松类成分测定及靶向筛查中的应用研究
- Author:
Shuo-Jun OU
1
;
Yin-Yin LIN
;
Hai-Tao ZHANG
;
Jian-Bin CEN
;
Zhi-Yuan WANG
;
Xin-Dong GUO
;
Jia-Jun ZHANG
;
Zhi-Sen LIANG
;
Guang-Feng ZENG
Author Information
1. 广州检验检测认证集团有限公司,国家加工食品质量检验中心(广东),广州 511447;广州质量监督检测研究院,国家加工食品质量监督检验中心(广东),广州 511447
- Keywords:
Feature ion guided strategy;
High resolution mass spectrometry;
Diflorasone;
Response surface design;
Health products
- From:
Chinese Journal of Analytical Chemistry
2025;53(8):1320-1330,中插88-中插92
- CountryChina
- Language:Chinese
-
Abstract:
A method for determination and targeted screening of diflorasone components in health products using ultra performance liquid chromatography-quadrupole time of flight mass spectrometry(UPLC-Q-TOF/MS)was established.Four representative diflorasone and esters(diflorasone,diflorasone diacetate,diflorasone-17-propionate,and diflorasone-21-propionate)were selected to optimize the pretreatment conditions,and 10 mL of extraction solvent dosage,15 min of extraction time and 5 g of salting-out agent as the optimal conditions were selected by response surface methodology.The results showed that the four analytes exhibited good linearity within the concentration range of 2.0?100 μg/L with the chromatographic peak area,and the correlation coefficients(R2)were all greater than 0.9990,while the results of recovery and relative standard deviation could satisfy the requirements of determination.The common characteristic ions of diflorasone and esters werem/z121 andm/z335,and their specific structures were obtained by analyzing the cleavage pathway based on the optimized determination conditions.A targeted screening method for other esters of diflorasone based on characteristic ions guidance strategy was established.This method had many advantages such as high efficiency,high sensitivity and good reproducibility,and could be used for targeted screening of diflorasone and esters in health products.The developed characteristic ion guided strategy could be employed to construct mass spectral databases for various glucocorticoids,enabling comprehensive targeted screening across a broad range of compounds.
