Mass Spectrometry-based Quantitative Metabolomics Analysis for Uncovering Metabolic Features in Myocardial Infarction Rats
10.19756/j.issn.0253-3820.251137
- VernacularTitle:基于质谱的定量代谢组学分析揭示心肌梗死大鼠的代谢特征
- Author:
Dan-Yang YU
1
;
Zi-Lin XIA
;
Na WANG
;
Hao WU
;
Lun-Zhao YI
Author Information
1. 昆明理工大学 食品科学与工程学院,昆明 650500
- Keywords:
Ultra-high performance liquid chromatography-mass spectrometry;
Metabolomics;
Lipidomics;
Myocardial infarction
- From:
Chinese Journal of Analytical Chemistry
2025;53(8):1311-1319,中插23-中插87
- CountryChina
- Language:Chinese
-
Abstract:
A quantitative analytical method was established for 275 kinds of metabolites in plasma based on ultra-high performance liquid chromatography-triple quadrupole mass spectrometry/multiple reaction monitoring-selected ion monitoring(UHPLC-QQQ-MS/MRM-SIM)technique.Based on UHPLC-high resolution(HRMS)/full MS-parallel reaction monitoring(PRM)technique,165 kinds of lipid metabolites in plasma were qualitatively identified and quantitatively detected to address the analytical challenges of lipid isomers.This quantitative metabolomics approach was successfully applied to a rat myocardial infarction study,identifying and quantifying 372 kinds of metabolites in total.Comparative analysis with the sham-operated group revealed significant alterations in 59 kinds of plasma metabolites in myocardial infarction rats.Key metabolic pathways,which included amino acid metabolism(alanine,aspartate,and glutamate metabolism;glycine,serine,and threonine metabolism,etc.),one-carbon unit metabolism,and tricarboxylic acid cycle,were significantly disrupted.
