High-sensitivity Ratio-type Surface-enhanced Raman Substrate for Rapid Quantitative Determination of 6-Thioguanine in Serum
10.19756/j.issn.0253-3820.251142
- VernacularTitle:高灵敏比率型表面增强拉曼基底用于快速分析血清中的6-硫鸟嘌呤
- Author:
Yan-Bin LIU
1
;
Yi-Chao HAN
;
Rong WANG
;
Xiao-Mei WU
;
Qin WANG
;
Yuan-Yuan YAO
;
Yue-Liang WANG
;
Long-Hua GUO
Author Information
1. 嘉兴大学生物与化学工程学院,嘉兴市分子识别材料与传感技术重点实验室,嘉兴 314001
- Keywords:
Ratio-type surface-enhanced Raman substrate;
Gold nanostars;
6-Thioguanine;
Anti-cancer drug
- From:
Chinese Journal of Analytical Chemistry
2025;53(8):1300-1310
- CountryChina
- Language:Chinese
-
Abstract:
6-Thioguanine(6-TG)is an antineoplastic agent used in treatment of acute leukemia.However,significant interindividual variability in dosing regimens and frequent clinical manifestations of hepatotoxicity and myelosuppression as adverse effects have affected its therapeutic efficacy.Consequently,the development of rapid analytical methods for 6-TG in clinical samples,enabling continuous therapeutic drug monitoring of plasma concentrations,holds substantial significance in optimizing dosage regimens,mitigating adverse reactions,and investigating drug metabolism mechanisms.In this study,multi-tipped gold nanostars(AuNSs)were prepared.With bis-(p-sulfonylphenyl)phenylphosphine molecule as the protecting agent and internal standard molecule,the AuNSs were assembled onto a highly sensitive surface-enhanced Raman(SERS)substrate for developing a ratio-based SERS quantitative analysis method for 6-TG in serum.The AuNSs containing multiple tips and gaps exhibited strong local surface plasmon resonance effect and SERS activity,ensuring the sensitivity of the analytical method.Furthermore,the introduction of internal standard molecules could improve the reproducibility,which guaranteed this method suitable for rapid analysis of drug molecules in complex samples.Quantitative analysis of 6-TG was achieved with linear detetion range of 1.0×10?4-1.0 mmol/L.In the spiked recovery experiments of serum,the RSD was less than 5.32%,and the recoveries were 94%-104%,which proved that this method could be used for rapid quantitative determination of 6-TG in serum.This method provided a powerful tool for studying drug pharmacokinetics,which could promote the optimization of the usage methods of anti-cancer drugs,and it was expected to further enhance the clinical efficacy and safety of 6-TG,enabling it to achieve the best therapeutic effect.
